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Distinction between the Cfr methyltransferase conferring antibiotic resistance and the housekeeping RlmN methyltransferase
- Source :
- Atkinson, G C, Hansen, L H, Tenson, T, Rasmussen, A, Kirpekar, F & Vester, B 2013, ' Distinction between the Cfr Methyltransferase Conferring Antibiotic Resistance and the Housekeeping RlmN Methyltransferase ', Antimicrobial Agents and Chemotherapy, vol. 57, no. 8, pp. 4019-4026 . https://doi.org/10.1128/AAC.00448-13
- Publication Year :
- 2013
-
Abstract
- The cfr gene encodes the Cfr methyltransferase that primarily methylates C-8 in A2503 of 23S rRNA in the peptidyl transferase region of bacterial ribosomes. The methylation provides resistance to six classes of antibiotics of clinical and veterinary importance. The rlmN gene encodes the RlmN methyltransferase that methylates C-2 in A2503 in 23S rRNA and A37 in tRNA, but RlmN does not significantly influence antibiotic resistance. The enzymes are homologous and use the same mechanism involving radical S -adenosyl methionine to methylate RNA via an intermediate involving a methylated cysteine in the enzyme and a transient cross-linking to the RNA, but they differ in which carbon atom in the adenine they methylate. Comparative sequence analysis identifies differentially conserved residues that indicate functional sequence divergence between the two classes of Cfr- and RlmN-like sequences. The differentiation between the two classes is supported by previous and new experimental evidence from antibiotic resistance, primer extensions, and mass spectrometry. Finally, evolutionary aspects of the distribution of Cfr- and RlmN-like enzymes are discussed.
- Subjects :
- Peptidyl transferase
Methyltransferase
Sequence analysis
Staphylococcus
Molecular Sequence Data
Microbial Sensitivity Tests
Biology
Ribosome
Methylation
Bacterial Proteins
23S ribosomal RNA
Sequence Analysis, Protein
Mechanisms of Resistance
Consensus Sequence
Drug Resistance, Bacterial
Consensus sequence
Escherichia coli
Pharmacology (medical)
Amino Acid Sequence
Databases, Protein
Phylogeny
Pharmacology
Genetics
Escherichia coli Proteins
Genetic Variation
Drug Resistance, Microbial
Methyltransferases
Ribosomal RNA
RNA, Bacterial
RNA, Ribosomal, 23S
Infectious Diseases
Biochemistry
Genes, Bacterial
Transfer RNA
biology.protein
Sequence Alignment
Plasmids
Subjects
Details
- ISSN :
- 10986596
- Volume :
- 57
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Antimicrobial agents and chemotherapy
- Accession number :
- edsair.doi.dedup.....79779d6641d2dd7a0ebd71e5d3fd8370