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MicroRNA expression patterns in canine mammary cancer show significant differences between metastatic and non-metastatic tumours
- Source :
- BMC Cancer, BMC Cancer, Vol 17, Iss 1, Pp 1-17 (2017)
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Background MicroRNAs may act as oncogenes or tumour suppressor genes, which make these small molecules potential diagnostic/prognostic factors and targets for anticancer therapies. Several common oncogenic microRNAs have been found for canine mammary cancer and human breast cancer. On account of this, large-scale profiling of microRNA expression in canine mammary cancer seems to be important for both dogs and humans. Methods Expression profiles of 317 microRNAs in 146 canine mammary tumours of different histological type, malignancy grade and clinical history (presence/absence of metastases) and in 25 control samples were evaluated. The profiling was performed using microarrays. Significance Analysis of Microarrays test was applied in the analysis of microarray data (both unsupervised and supervised data analyses were performed). Validation of the obtained results was performed using real-time qPCR. Subsequently, predicted targets for the microRNAs were searched for in miRBase. Results Results of the unsupervised analysis indicate that the primary factor separating the samples is the metastasis status. Predicted targets for microRNAs differentially expressed in the metastatic vs. non-metastatic group are mostly engaged in cell cycle regulation, cell differentiation and DNA-damage repair. On the other hand, the supervised analysis reveals clusters of differentially expressed microRNAs unique for the tumour type, malignancy grade and metastasis factor. Conclusions The most significant difference in microRNA expression was observed between the metastatic and non-metastatic group, which suggests a more important role of microRNAs in the metastasis process than in the malignant transformation. Moreover, the differentially expressed microRNAs constitute potential metastasis markers. However, validation of cfa-miR-144, cfa-miR-32 and cfa-miR-374a levels in blood samples did not follow changes observed in the non-metastatic and metastatic tumours. Electronic supplementary material The online version of this article (10.1186/s12885-017-3751-1) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Cancer Research
Pathology
Unclassified drug
Real time polymerase chain reaction
Animal tissue
MiRBase
Metastasis
Malignant transformation
Breast cancer
0302 clinical medicine
Surgical oncology
Cell differentiation
Dog
Gene Regulatory Networks
Dog Diseases
cfa miR 374a
Neoplasm Metastasis
Oligonucleotide Array Sequence Analysis
microRNA
Tumor classification
MicroRNA
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Gene Expression Regulation, Neoplastic
Oncology
030220 oncology & carcinogenesis
Significance analysis of microarrays
Female
DNA microarray
Research Article
medicine.medical_specialty
DNA repair
Mammary Neoplasms, Animal
lcsh:RC254-282
Cancer grading
03 medical and health sciences
Dogs
Genetics
medicine
Animals
cfa miR 144
Human breast cancer
business.industry
Microarray analysis techniques
Gene Expression Profiling
Microarray analysis
Canine mammary cancer
Nonhuman
medicine.disease
MicroRNAs
030104 developmental biology
Cancer research
cfa miR 32
Gene expression
Cell cycle regulation
business
Controlled study
Subjects
Details
- ISSN :
- 14712407
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- BMC Cancer
- Accession number :
- edsair.doi.dedup.....7985cd43b3b2e4d9db94cd54e4e8ecbb