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Hyperbaric oxygen via mediating SIRT1‐induced deacetylation of HMGB1 improved cReperfusion inj/reperfusion injury
- Source :
- European Journal of Neuroscience. 54:7318-7331
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Ischemic stroke leads to severe neurological dysfunction in adults. Hyperbaric oxygen (HBO) induces tolerance to cReperfusion inj/reperfusion (I/R) injury. Therefore, our aims were to investigate whether SIRT1 participates in regulatingin the neuro-protective effect of HBO in a cerebral I/R model and its mechanism. Mice N2a cells were used to construct an oxygen deprivation/reperfusion (OGD/R) model to simulate in vitro brain I/R injury and to evaluate the role of HBO in OGD/R stimulated cells. Cell proliferation was detected using MTT, and apoptosis was determined by flow cytometry. ELISA was used to measure the concentration of TNF-α, IL-1β and IL-6 related inflammatory factors. RT-qPCR and western blot assays were performed to test the expression of SIRT1. Immunoprecipitation was used to detect acetylation of HMGB1. Expression of SIRT1 was obviously reduced after OGD/R treatment in N2a cells, while SIRT1 was obviously enhanced in HBO treated cells. Moreover, knockdown of SIRT1 induced neuro-inflammation damage in cells and HBO effectively improved the inflammatory response in OGD/R treated cells by affecting SIRT1 levels. Furthermore, HBO induced the deacetylation of HMGB1 via regulating SIRT1. Interestingly, HBO via regulating the SIRT1-induced HMGB1 deacetylation and suppressing MMP-9 improved ischemic brain injury. HBO regulated ischemic brain injury via regulation of SIRT1-induced HMGB1 deacetylation, making it a potential treatment for ischemic brain injury treatment.
- Subjects :
- Hyperbaric Oxygenation
Gene knockdown
medicine.diagnostic_test
biology
Cell growth
business.industry
General Neuroscience
Pharmacology
medicine.disease
HMGB1
In vitro
Flow cytometry
Oxygen
Mice
Sirtuin 1
Western blot
Apoptosis
Reperfusion Injury
medicine
biology.protein
Animals
HMGB1 Protein
business
Reperfusion injury
Subjects
Details
- ISSN :
- 14609568 and 0953816X
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- European Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....79904eacae0af16704dbd6a5e1ba3746
- Full Text :
- https://doi.org/10.1111/ejn.15458