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Carnosic acid impedes cell growth and enhances anticancer effects of carmustine and lomustine in melanoma
- Source :
- Bioscience Reports
- Publication Year :
- 2018
- Publisher :
- Portland Press Ltd., 2018.
-
Abstract
- Carnosic acid (CA), a major polyphenolic diterpene present in Rosmarinus officinalis, has been reported to have multiple functions, including antitumor activity. The MTT assay, BrdU incorporation, wound healing, and colony formation were used to detect melanoma B16F10 cell growth and proliferation. Flow cytometry was used for cell cycle detection. p21 and p27 expression was detected by Western blotting. B16F10 cell xenograft model was established, and treated with CA, carmustine (BCNU), or lomustine (CCNU). The present study found that CA exhibits significant growth inhibition and cell cycle arrest in melanoma B16F10 cells. We also found that CA triggers cell cycle arrest at G0/G1 phase, and enhances p21 expression. Additionally, CA can enhance BCNU- and CCNU-mediated cytotoxicity and cell cycle arrest in B16F10 cells. Finally, we found that CA inhibits tumor growth, and reduces the values of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in vivo. The present study study concluded that CA may be safe and useful as a novel chemotherapeutic agent.
- Subjects :
- 0301 basic medicine
Male
Cell cycle checkpoint
lomustine
Biophysics
Melanoma, Experimental
Apoptosis
Biochemistry
Antioxidants
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Cell Line, Tumor
medicine
Animals
MTT assay
Molecular Biology
carnosic acid
Antineoplastic Agents, Alkylating
Research Articles
Cell Proliferation
Carmustine
carmustine
Chemistry
Cell growth
B16F10
Carnosic acid
Drug Synergism
Cell Biology
Lomustine
Cell Cycle Checkpoints
Cell cycle
Mice, Inbred C57BL
030104 developmental biology
030220 oncology & carcinogenesis
Abietanes
Cancer research
cell cycle
Growth inhibition
medicine.drug
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 15734935 and 01448463
- Volume :
- 38
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Bioscience Reports
- Accession number :
- edsair.doi.dedup.....79ca330018f08b534ae303e8bd29a4ba