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A ready-to-use dry powder formulation based on protamine nanocarriers for pulmonary drug delivery
- Source :
- European Journal of Pharmaceutical Sciences. 185:106442
- Publication Year :
- 2023
- Publisher :
- Elsevier BV, 2023.
-
Abstract
- The use of oral antibiotic therapy for the treatment of respiratory diseases as tuberculosis has promoted the appearance of side effects as well as resistance to these treatments. The low solubility, high metabolism, and degradation of drugs as rifabutin, have led to the use of combined and prolonged therapies, which difficult patient compliance. In this work, we develop inhalable formulations from biomaterials such as protamine to improve the therapeutic effect. Rifabutin-loaded protamine nanocapsules (NCs) were prepared by solvent displacement method and were physico-chemically characterized and evaluated for their dissolution, permeability, stability, cytotoxicity, hemocompatibility, internalization, and aerodynamic characteristics after a spray-drying procedure. Protamine NCs presented a size of around 200 nm, positive surface charge, and drug association up to 54%. They were stable as suspension under storage, as well as in biological media and as a dry powder after lyophilization in the presence of mannitol. Nanocapsules showed a good safety profile and cellular uptake with no tolerogenic effect on macrophages and showed good compatibility with red blood cells. Moreover, the aerodynamic evaluation showed a fine particle fraction deposition up to 30% and a mass median aerodynamic diameter of about 5 µm, suitable for the pulmonary delivery of therapeutics This research was funded by Ministerio de Ciencia e Innovacion RETOS - PID2019-107500RB-I00, Ministry of Human Capacities, Hungary grant TKP2021-EGA-32 and the Erasmus+ program of the European Union SI
- Subjects :
- Protamine nanocapsules
Anti-tuberculotic treatment
eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107500RB-I00/ES/BIOMORPHIC PLATFORMS FOR THE PULMONARY DELIVERY OF NANOTHERAPEUTICS [info]
Rifabutin
Pharmaceutical Science
03.01. Általános orvostudomány
Mannitol
Pulmonary drug delivery
Subjects
Details
- ISSN :
- 09280987
- Volume :
- 185
- Database :
- OpenAIRE
- Journal :
- European Journal of Pharmaceutical Sciences
- Accession number :
- edsair.doi.dedup.....7a35c254ba9244e5a7907b16c49ad8cb
- Full Text :
- https://doi.org/10.1016/j.ejps.2023.106442