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Preparation and in vivo efficient anti-infection property of GTR/GBR implant made by metronidazole loaded electrospun polycaprolactone nanofiber membrane
- Source :
- International Journal of Pharmaceutics. 475:566-577
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Infection is the major reason of GTR/GBR membrane failure in clinical application. In this work, we developed GTR/GBR nanofiber membranes with localized drug delivery function to prevent infection. Metronidazole (MNA), an antibiotic, was successfully incorporated into electrospun polycaprolactone (PCL) nanofibers at different concentrations (0, 1, 5, 10, 20, 30, and 40 wt% polymer). To obtain the optimum anti-infection membrane, we systematically investigated the physical-chemical and mechanical properties of the nanofiber membranes with different drug contents. The interaction between PCL and MNA was identified by molecular dynamics simulation. MNA released in a controlled, sustained manner over 2 weeks and the antibacterial activity of the released MNA remained. The incorporation of MNA improved the hydrophilicity and in vitro biodegradation rate of PCL nanofibers. The nanofiber membranes allowed cells to adhere to and proliferate on them and showed excellent barrier function. The membrane loaded with 30% MNA had the best comprehensive properties. Analysis of subcutaneous implants demonstrated that MNA-loaded nanofibers evoked a less severe inflammatory response than pure PCL nanofibers. These results demonstrate the potential of MNA-loaded nanofiber membranes as GTR/GBR membrane with antibacterial and anti-inflammatory function for extensive biomedical applications.
- Subjects :
- Male
Microbiological Techniques
Bone Regeneration
Nanofibers
Pharmaceutical Science
Biocompatible Materials
chemistry.chemical_compound
Drug Delivery Systems
Anti-Infective Agents
In vivo
Metronidazole
Animals
Caprolactam
Cell Proliferation
Dose-Response Relationship, Drug
Guided Tissue Regeneration
Prostheses and Implants
Fibroblasts
Electrospinning
Drug Liberation
Membrane
chemistry
Nanofiber
Polycaprolactone
Drug delivery
Rabbits
Implant
Antibacterial activity
Biomedical engineering
Subjects
Details
- ISSN :
- 03785173
- Volume :
- 475
- Database :
- OpenAIRE
- Journal :
- International Journal of Pharmaceutics
- Accession number :
- edsair.doi.dedup.....7a361cb5d02b2fc0a710eb948198135c