Back to Search
Start Over
Profiling Glioblastoma Cases with an Expression of DCX, OLIG2 and NES
- Source :
- International Journal of Molecular Sciences; Volume 22; Issue 24; Pages: 13217, International Journal of Molecular Sciences, Vol 22, Iss 13217, p 13217 (2021), International Journal of Molecular Sciences
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Glioblastoma (GBM) remains the leading cause of cancer-related deaths with the lowest five-year survival rates among all of the human cancers. Multiple factors contribute to its poor outcome, including intratumor heterogeneity, along with migratory and invasive capacities of tumour cells. Over the last several years Doublecortin (DCX) has been one of the debatable factors influencing GBM cells’ migration. To resolve DCX’s ambiguous role in GBM cells’ migration, we set to analyse the expression patterns of DCX along with Nestin (NES) and Oligodendrocyte lineage transcription factor 2 (OLIG2) in 17 cases of GBM, using immunohistochemistry, followed by an analysis of single-cell RNA-seq data. Our results showed that only a small subset of DCX positive (DCX+) cells was present in the tumour. Moreover, no particular pattern emerged when analysing DCX+ cells relative position to the tumour margin. By looking into single-cell RNA-seq data, the majority of DCX+ cells were classified as non-cancerous, with a small subset of cells that could be regarded as glioma stem cells. In conclusion, our findings support the notion that glioma cells express DCX; however, there is no clear evidence to prove that DCX participates in GBM cell migration.
- Subjects :
- Doublecortin Protein
QH301-705.5
OLIG2
Doublecortin
Article
Catalysis
Nestin
Inorganic Chemistry
Cell Movement
Image Processing, Computer-Assisted
Heuristics
Humans
single cell RNA-seq
Neoplasm Metastasis
immunofluorescence
Biology (General)
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Spectroscopy
Microscopy, Confocal
Glioblastoma
DCX
NES
immunohistochemistry
brain tumours
Brain Neoplasms
Sequence Analysis, RNA
Gene Expression Profiling
Organic Chemistry
General Medicine
Oligodendrocyte Transcription Factor 2
Survival Analysis
Computer Science Applications
Gene Expression Regulation, Neoplastic
Chemistry
Neoplastic Stem Cells
Single-Cell Analysis
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences; Volume 22; Issue 24; Pages: 13217
- Accession number :
- edsair.doi.dedup.....7a3c86dc0c7fa845bd65e7401105bc39
- Full Text :
- https://doi.org/10.3390/ijms222413217