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Profiling Glioblastoma Cases with an Expression of DCX, OLIG2 and NES

Authors :
Adrian Odrzywolski
Bożena Jarosz
Michał Kiełbus
Ilona Telejko
Dominik Ziemianek
Sebastian Knaga
Radosław Rola
Source :
International Journal of Molecular Sciences; Volume 22; Issue 24; Pages: 13217, International Journal of Molecular Sciences, Vol 22, Iss 13217, p 13217 (2021), International Journal of Molecular Sciences
Publication Year :
2021
Publisher :
Multidisciplinary Digital Publishing Institute, 2021.

Abstract

Glioblastoma (GBM) remains the leading cause of cancer-related deaths with the lowest five-year survival rates among all of the human cancers. Multiple factors contribute to its poor outcome, including intratumor heterogeneity, along with migratory and invasive capacities of tumour cells. Over the last several years Doublecortin (DCX) has been one of the debatable factors influencing GBM cells’ migration. To resolve DCX’s ambiguous role in GBM cells’ migration, we set to analyse the expression patterns of DCX along with Nestin (NES) and Oligodendrocyte lineage transcription factor 2 (OLIG2) in 17 cases of GBM, using immunohistochemistry, followed by an analysis of single-cell RNA-seq data. Our results showed that only a small subset of DCX positive (DCX+) cells was present in the tumour. Moreover, no particular pattern emerged when analysing DCX+ cells relative position to the tumour margin. By looking into single-cell RNA-seq data, the majority of DCX+ cells were classified as non-cancerous, with a small subset of cells that could be regarded as glioma stem cells. In conclusion, our findings support the notion that glioma cells express DCX; however, there is no clear evidence to prove that DCX participates in GBM cell migration.

Details

Language :
English
ISSN :
14220067
Database :
OpenAIRE
Journal :
International Journal of Molecular Sciences; Volume 22; Issue 24; Pages: 13217
Accession number :
edsair.doi.dedup.....7a3c86dc0c7fa845bd65e7401105bc39
Full Text :
https://doi.org/10.3390/ijms222413217