Additional file 1: of Early myeloid-derived suppressor cells (HLA-DR−/lowCD33+CD16−) expanded by granulocyte colony-stimulating factor prevent acute graft-versus-host disease (GVHD) in humanized mouse and might contribute to lower GVHD in patients post allo-HSCT

Table S1. Cell components in graft. Figure S1. HLA-DR−/lowCD33+CD16− eMDSCs demonstrated superior immune-suppressive activity compared with CD33− and HLA-DR−/lowCD33+CD16+ fraction. Figure S2. Comparison of immune-suppressive activity of HLA-DR−/lowCD33+CD16− eMDSCs with M-MDSCs and G-MDSCs. Figure S3. Selective inhibitors of arginase (nor-NOHA), iNOS (L-NMMA), and IDO (NLG8189) on T cell proliferation. Figure S4. Human white blood cell engraftment at 7 days, 14 days, and 21 days after co-transplantation. Figure S5. eMDSC engraftment in NSG mice at 14 and 21 days after co-transplantation. Figure S6. Treg, Th1, Th2, and Th17 cells detected in the peripheral blood in NSG mice at day 21. Figure S7. Cytokines detected in the peripheral blood of NSG mice at 7, 14, and 21 days after co-transplantation. (DOCX 724 kb)