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Characterization of a novel disease resistance gene rtp3 and its association with VNN disease resistance in Asian seabass
- Source :
- Fish & Shellfish Immunology. 61:61-67
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Asian seabass, an important food fish in Southeast Asia, has suffered from nervous necrosis virus (NNV) infection, resulting in massive mortality of Asian seabass larvae and enormous economic losses. Identification and characterization of disease resistance genes is important. Previous transcriptome analysis of Asians seabass epithelial cells after NNV infection revealed a highly inducible gene, receptor-transporting protein 3 ( rtp3 ), indicating it could play an important role in Asian seabass – NNV interaction. To characterize this gene, we determined its expression pattern and subcellular localization. The rtp3 was highly induced in most examined tissues and organs of Asian seabass after NNV infection, and protein Rtp3 was localized in cytoplasm. Further association study in multiple families revealed that a microsatellite marker, (GT)ntt(GT)n, in the 3′ UTR of rtp3 was significantly associated with VNN disease resistance in Asian seabass. Our results imply that rtp3 may be a novel disease resistance gene in Asian seabass. This data could improve our understanding of molecular interaction between Asian seabass and NNV, and has the potential to be applied in marker-assisted selection for disease resistance breeding in Asian seabass.
- Subjects :
- 0301 basic medicine
Untranslated region
Gene Expression
Aquatic Science
Plant disease resistance
Biology
Virus
Transcriptome
Fish Diseases
03 medical and health sciences
RNA Virus Infections
0302 clinical medicine
Expression pattern
Animals
Environmental Chemistry
Nodaviridae
Asian seabass
Gene
Disease Resistance
Genetics
Gene Expression Profiling
General Medicine
Perciformes
030104 developmental biology
030220 oncology & carcinogenesis
Microsatellite
Microsatellite Repeats
Subjects
Details
- ISSN :
- 10504648
- Volume :
- 61
- Database :
- OpenAIRE
- Journal :
- Fish & Shellfish Immunology
- Accession number :
- edsair.doi.dedup.....7a652d78ca914694e5e068dcf6ebe0c7