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Establishment of a Cre-rat resource for creating conditional and physiological relevant models of human diseases
- Source :
- Transgenic Research
- Publication Year :
- 2020
-
Abstract
- The goal of this study is to establish a Cre/loxP rat resource for conditional and physiologically predictive rat models of human diseases. The laboratory rat (R. norvegicus) is a central experimental animal in several fields of biomedical research, such as cardiovascular diseases, aging, infectious diseases, autoimmunity, cancer models, transplantation biology, inflammation, cancer risk assessment, industrial toxicology, pharmacology, behavioral and addiction studies, and neurobiology. Up till recently, the ability of creating genetically modified rats has been limited compared to that in the mouse mainly due to lack of genetic manipulation tools and technologies in the rat. Recent advances in nucleases, such as CRISPR/Cas9 (clustered regularly-interspaced short palindromic repeats/CRISPR associated protein 9), as well as TARGATTâ„¢ integrase system enables fast, efficient and site-specific introduction of exogenous genetic elements into the rat genome. Here, we report the generation of a collection of tissue-specific, inducible transgenic Cre rats as tool models using TARGATTâ„¢, CRISPR/Cas9 and random transgenic approach. More specifically, we generated Cre driver rat models that allow controlled gene expression or knockout (conditional models) both temporally and spatially through the Cre-ERT2/loxP system. A total of 10 Cre rat lines and one Cre reporter/test line were generated, including eight (8) Cre lines for neural specific and two (2) lines for cardiovascular specific Cre expression. All of these lines have been deposited with the Rat Resource and Research Center and provide a much-needed resource for the bio-medical community who employ rat models for their studies of human diseases.
- Subjects :
- 0301 basic medicine
Aging
Transgene
Autoimmunity
Computational biology
Genome
Communicable Diseases
03 medical and health sciences
0302 clinical medicine
Neoplasms
Genetics
CRISPR
Animals
Humans
TARGATT
CRISPR/Cas9
Inflammation
Transplantation
Original Paper
biology
Integrases
Cas9
Genetic Diseases, Inborn
Cre
Integrase
Genetically modified organism
Laboratory rat
Rats
Transgenic rat
Disease Models, Animal
030104 developmental biology
Cardiovascular Diseases
biology.protein
Animal Science and Zoology
CRISPR-Cas Systems
Agronomy and Crop Science
030217 neurology & neurosurgery
Biotechnology
Subjects
Details
- ISSN :
- 15739368
- Volume :
- 30
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Transgenic research
- Accession number :
- edsair.doi.dedup.....7a7ee99c86e764dcebd6747f5c4c1a41