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Identification of a DNA Nonhomologous End-Joining Complex in Bacteria
- Source :
- Science. 297:1686-1689
- Publication Year :
- 2002
- Publisher :
- American Association for the Advancement of Science (AAAS), 2002.
-
Abstract
- In eukaryotic cells, double-strand breaks (DSBs) in DNA are generally repaired by the pathway of homologous recombination or by DNA nonhomologous end joining (NHEJ). Both pathways have been highly conserved throughout eukaryotic evolution, but no equivalent NHEJ system has been identified in prokaryotes. The NHEJ pathway requires a DNA end-binding component called Ku. We have identified bacterial Ku homologs and show that these proteins retain the biochemical characteristics of the eukaryotic Ku heterodimer. Furthermore, we show that bacterial Ku specifically recruits DNA ligase to DNA ends and stimulates DNA ligation. Loss of these proteins leads to hypersensitivity to ionizing radiation in Bacillus subtilis . These data provide evidence that many bacteria possess a DNA DSB repair apparatus that shares many features with the NHEJ system of eukarya and suggest that this DNA repair pathway arose before the prokaryotic and eukaryotic lineages diverged.
- Subjects :
- DNA, Bacterial
Ku80
DNA Ligases
DNA Repair
DNA repair
Biology
Bacterial Proteins
Ku Autoantigen
Replication protein A
Genetics
chemistry.chemical_classification
DNA ligase
Binding Sites
Multidisciplinary
fungi
DNA Helicases
Nuclear Proteins
Antigens, Nuclear
DNA Repair Pathway
DNA repair protein XRCC4
DNA-Binding Proteins
Non-homologous end joining
enzymes and coenzymes (carbohydrates)
chemistry
Mutation
DNA polymerase mu
Bacillus subtilis
DNA Damage
Protein Binding
Subjects
Details
- ISSN :
- 10959203 and 00368075
- Volume :
- 297
- Database :
- OpenAIRE
- Journal :
- Science
- Accession number :
- edsair.doi.dedup.....7a82ed73af1050cd9eca2d18a07eeaaf