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Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF-β signaling pathway
- Source :
- Molecular medicine reports. 10(4)
- Publication Year :
- 2013
-
Abstract
- The transforming growth factor-β (TGF-β) signaling pathway exhibits an important role in cancer invasion and metastasis. Excessive expression of TGF-β activates Smad4, leading to the upregulation of downstream metastasis-associated genes. Thus, the inhibition of the TGF-β/Smad4 signaling pathway may be a novel strategy for treatment of cancer metastasis. Baicalein, a flavonoid derived from the root of Scutellaria baicalensis, has been reported to exert strong anti-tumor activity towards various types of cancer. In the present study the effect of baicalein on migration and invasion of cancer cells was evaluated using wound-healing and Transwell assays. In order to investigate the possible molecular mechanisms of the anti-metastatic effects of baicalein, quantitative polymerase chain reaction (qPCR) and western blot analyses were performed to examine the effect on the expression of TGF‑β, Smad4, N-cadherin, vimentin, ZEB1 and ZEB2. It was determined that baicalein inhibited the migration and invasion of AGS cells by suppressing the TGF-β/Smad4 signaling pathway. In addition, baicalein treatment reduced the expression of the metastasis-associated N-cadherin, vimentin, ZEB1 and ZEB2, downstream target genes of the TGF‑β/Smad4 signaling pathway. Collectively, these results suggest that inhibition of the metastasis of cancer cells via inactivation of TGF-β/Smad4 signaling is one of the mechanisms by which baicalein may treat cancer.
- Subjects :
- Cancer Research
Cell
Biochemistry
Plant Roots
Metastasis
chemistry.chemical_compound
Cell Movement
Stomach Neoplasms
Transforming Growth Factor beta
Cell Line, Tumor
Genetics
medicine
Humans
Vimentin
Molecular Biology
Smad4 Protein
Zinc Finger E-box Binding Homeobox 2
Homeodomain Proteins
Oncogene
biology
Cancer
Zinc Finger E-box-Binding Homeobox 1
medicine.disease
biology.organism_classification
Cadherins
Antineoplastic Agents, Phytogenic
Baicalein
Cell biology
Repressor Proteins
medicine.anatomical_structure
Oncology
chemistry
Cancer cell
Flavanones
Molecular Medicine
Scutellaria baicalensis
Signal transduction
Signal Transduction
Transcription Factors
Subjects
Details
- ISSN :
- 17913004
- Volume :
- 10
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Molecular medicine reports
- Accession number :
- edsair.doi.dedup.....7a86420be36ce781c03a6cb648f939bb