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Maximizing Tandem Mass Spectrometry Acquisition Rates for Shotgun Proteomics

Authors :
Dain R. Brademan
Joshua J. Coon
Edna A. Trujillo
Alexander S. Hebert
Source :
Anal Chem
Publication Year :
2019
Publisher :
American Chemical Society (ACS), 2019.

Abstract

Advances in tandem mass spectrometry (MS/MS) acquisition rate have steadily led to increased performance in shotgun proteomics experiments. To that end, contemporary mass spectrometers are outfitted with multiple analyzers allowing for the simultaneous collection of survey (MS1) and MS/MS spectra. In the latest generation Orbitrap hybrid, MS/MS scans can be acquired at a high rate using the dual cell linear ion trap analyzer, all while the next precursor is being dissociated in a collision cell and a MS1 scan is occurring in the Orbitrap. Often overlooked in these experiments is that the ion trap scan duration is highly variable and dependent upon precursor mass. Here, we examine the use of various static mass-to-charge ratio scan ranges for ion trap MS/MS acquisition and determine performance relative to conventional dynamic mass-to-charge ratio range scanning. We demonstrate that a fixed mass-to-charge ratio scan range can generate 12% more MS/MS scans and more unique peptide identifications as compared to the standard dynamic approach, respectively.

Details

ISSN :
15206882 and 00032700
Volume :
91
Database :
OpenAIRE
Journal :
Analytical Chemistry
Accession number :
edsair.doi.dedup.....7aa4462bb6632f0e7ec566100f42f4ff
Full Text :
https://doi.org/10.1021/acs.analchem.9b02979