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Connexin 36 Mediates Orofacial Pain Hypersensitivity Through GluK2 and TRPA1
- Source :
- Neurosci Bull
- Publication Year :
- 2020
- Publisher :
- Springer Singapore, 2020.
-
Abstract
- Trigeminal neuralgia is a debilitating condition, and the pain easily spreads to other parts of the face. Here, we established a mouse model of partial transection of the infraorbital nerve (pT-ION) and found that the Connexin 36 (Cx36) inhibitor mefloquine caused greater alleviation of pT-ION-induced cold allodynia compared to the reduction of mechanical allodynia. Mefloquine reversed the pT-ION-induced upregulation of Cx36, glutamate receptor ionotropic kainate 2 (GluK2), transient receptor potential ankyrin 1 (TRPA1), and phosphorylated extracellular signal regulated kinase (p-ERK) in the trigeminal ganglion. Cold allodynia but not mechanical allodynia induced by pT-ION or by virus-mediated overexpression of Cx36 in the trigeminal ganglion was reversed by the GluK2 antagonist NS102, and knocking down Cx36 expression in Nav1.8-expressing nociceptors by injecting virus into the orofacial skin area of Nav1.8-Cre mice attenuated cold allodynia but not mechanical allodynia. In conclusion, we show that Cx36 contributes greatly to the development of orofacial pain hypersensitivity through GluK2, TRPA1, and p-ERK signaling. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12264-020-00594-4) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Male
Orofacial pain
China
Indoles
Physiology
Kainate receptor
Pharmacology
Connexins
03 medical and health sciences
chemistry.chemical_compound
Infraorbital nerve
Trigeminal ganglion
Mice
0302 clinical medicine
Receptors, Kainic Acid
Trigeminal neuralgia
Facial Pain
Oximes
medicine
Animals
TRPA1 Cation Channel
business.industry
General Neuroscience
General Medicine
medicine.disease
nervous system diseases
Cold Temperature
Mefloquine
Mice, Inbred C57BL
030104 developmental biology
Allodynia
NS102
chemistry
Trigeminal Ganglion
Hyperalgesia
Nociceptor
Original Article
sense organs
medicine.symptom
business
psychological phenomena and processes
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Neurosci Bull
- Accession number :
- edsair.doi.dedup.....7ab6a29824afc8e0c1f3b9c2507f9cf4