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Combined analysis of plasma or serum glucosylsphingosine and globotriaosylsphingosine by UPLC-MS/MS
- Source :
- Clinica chimica acta; international journal of clinical chemistry. 511
- Publication Year :
- 2020
-
Abstract
- Purpose To develop a method for the combined analysis of plasma and serum glucosylsphingosine (lyso-Gb1) and globotriaosylsphingosine (lyso-Gb3), biomarkers of Gaucher disease (GD) and Fabry disease (FD), respectively. Methods Internal standards were added to 100 µL of plasma/serum and glycosphingolipids: lyso-Gb1, lyso-Gb3, and galactosylsphingosine (GalSph) were extracted with dichloromethane/methanol and analyzed by UPLC-MS/MS. Samples from unaffected controls and patients with GD were first analyzed using a HILIC column to separate lyso-Gb1 from its isomer, GalSph. Samples from patients with FD or GD were analyzed using a C18 column to measure lyso-Gb3 and the hexosylsphingosine (HexSph: lyso-Gb1 + GalSph) fraction in a single combined method. Results Extraction efficiency was between 73% and 87% and day-to-day variability showed a relative standard error of Conclusions HexSph and lyso-Gb3 analysis by reversed-phase chromatography UPLC-MS/MS is a cost-effective, time-efficient approach for evaluating these glycosphingolipid biomarkers in patients with a suspected or confirmed diagnosis of GD and FD.
- Subjects :
- 0301 basic medicine
Clinical Biochemistry
Biochemistry
High-performance liquid chromatography
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Tandem Mass Spectrometry
medicine
Humans
In patient
Combined method
Chromatography, High Pressure Liquid
Sphingolipids
Chromatography
Chemistry
Hydrophilic interaction chromatography
Biochemistry (medical)
Psychosine
General Medicine
Glycosphingolipid
respiratory system
medicine.disease
Fabry disease
030104 developmental biology
030220 oncology & carcinogenesis
Fabry Disease
lipids (amino acids, peptides, and proteins)
Uplc ms ms
Glycolipids
Biomarkers
Chromatography, Liquid
Subjects
Details
- ISSN :
- 18733492
- Volume :
- 511
- Database :
- OpenAIRE
- Journal :
- Clinica chimica acta; international journal of clinical chemistry
- Accession number :
- edsair.doi.dedup.....7acb0e2b3dcf772a006ccc1068beee98