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Study of Stem Cells Influence on Cardiac Cells Cultured with a Cyanide-P-Trifluoromethoxyphenylhydrazone in Organ-on-a-Chip System

Authors :
Dominik Kolodziejek
Tomasz Ciach
Anna Kobuszewska
Elzbieta Jastrzebska
Zbigniew Brzozka
Michał Wojasiński
Source :
Biosensors, Biosensors, Vol 11, Iss 131, p 131 (2021), Volume 11, Issue 5
Publication Year :
2021

Abstract

Regenerative medicine and stem cells could prove to be an effective solution to the problem of treating heart failure caused by ischemic heart disease. However, further studies on the understanding of the processes which occur during the regeneration of damaged tissue are needed. Microfluidic systems, which provide conditions similar to in vivo, could be useful tools for the development of new therapies using stem cells. We investigated how mesenchymal stem cells (MSCs) affect the metabolic activity of cardiac cells (rat cardiomyoblasts and human cardiomyocytes) incubated with a potent uncoupler of mitochondrial oxidative phosphorylation under microfluidic conditions. A cyanide p-trifluoromethoxyphenylhydrazone (FCCP) was used to mimic disfunctions of mitochondria of cardiac cells. The study was performed in a microfluidic system integrated with nanofiber mats made of poly-l-lactid acid (PLLA) or polyurethane (PU). The microsystem geometry allows four different cell cultures to be conducted under different conditions (which we called: normal, abnormal—as both a mono- and co-culture). Metabolic activity of the cells, based on the bioluminescence assay, was assessed in the culture’s performed in the microsystem. It was proved that stem cells increased metabolic activity of cardiac cells maintained with FCCP.

Details

ISSN :
20796374
Volume :
11
Issue :
5
Database :
OpenAIRE
Journal :
Biosensors
Accession number :
edsair.doi.dedup.....7ad139d5c7ddfbca4599898f3a512149