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Regulation of cardiac CACNB2 by microRNA-499: Potential role in atrial fibrillation
- Source :
- BBA Clinical
- Publication Year :
- 2017
- Publisher :
- Elsevier, 2017.
-
Abstract
- The L-type calcium channel (LTCC) is one of the major ion channels that are known to be associated with the electrical remodeling of atrial fibrillation (AF). In AF, there is significant downregulation of the LTCC, but the underlying mechanism for such downregulation is not clear. We have previously reported that microRNA-499 (miR-499) is significantly upregulated in patients with permanent AF and that KCNN3, the gene that encodes the small-conductance calcium-activated potassium channel 3 (SK3), is a target of miR-499. We found that CACNB2, an important subunit of the LTCC, is also a target of miR-499. We hypothesize that miR-499 plays an important role in AF electrical remodeling by regulating the expression of CACNB2 and the LTCC. In atrial tissue from patients with permanent AF, CACNB2 was significantly downregulated by 67% (n = 4, p<br />Highlights • LTCC is downregulated with electrical remodeling of atrial fibrillation. • MiR-499 is increased and CACNB2 is reduced in atria from patients with atrial fibrillation. • MiR-499 binds to the 3′UTR of CACNB2 and inhibits its protein expression. • Downregulation of CACNB2 results in the downregulation of LTCC pore-forming subunit. • MiR-499 contributes to the electrical remodeling of AF through regulation of CACNB2.
- Subjects :
- 0301 basic medicine
AF, atrial fibrillation
CACNB2, voltage-dependent calcium channel β-2 subunit
miRISC, microRNA-inducing silencing complex
030204 cardiovascular system & hematology
Biology
Ago, Argonaute
Pathology and Forensic Medicine
03 medical and health sciences
0302 clinical medicine
SK3
Downregulation and upregulation
Physiology (medical)
microRNA
Gene silencing
SR, sinus rhythm
Messenger RNA
Calcium channel
LTCC, L-type calcium channel
Regular Article
Transfection
Molecular biology
Potassium channel
Cell biology
UTR, untranslated region
030104 developmental biology
GAPDH, glyceraldehyde-3-phosphate dehydrogenase
Molecular Medicine
miR-499, microRNA-499
CACNA1C, voltage-dependent calcium channel α-1C subunit
Subjects
Details
- Language :
- English
- ISSN :
- 22146474
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- BBA Clinical
- Accession number :
- edsair.doi.dedup.....7ad206c2f9f3ade1e026a2b3774fb426