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Production of polyclonal antiserum specific to the 27.5 kDa envelope protein of white spot syndrome virus
- Source :
- Diseases of Aquatic Organisms, 51(1), 77-80, Diseases of Aquatic Organisms 51 (2002) 1
- Publication Year :
- 2002
-
Abstract
- A truncated version of the white spot syndrome virus (WSSV) 27.5 kDa envelope protein was expressed as a histidine tag fusion protein in Escherichia coli. The bacterial expression system allowed the production of up to 10 mg of purified recombinant protein per liter of bacterial culture. Antiserum from a rabbit immunized with the recombinant protein recognized the 27.5 kDa viral envelope protein of WSSV isolated from different geographic regions. The antiserum did not recognize any of the other known WSSV structural proteins. A sensitive immunodot assay for WSSV was developed using the specific rabbit polyclonal antiserum.
- Subjects :
- White spot syndrome
Immunoblotting
Laboratory of Virology
Aquaculture
Aquatic Science
medicine.disease_cause
Antibodies, Viral
Polymerase Chain Reaction
Virus
law.invention
Laboratorium voor Virologie
monodon
Viral envelope
Penaeidae
Viral Envelope Proteins
law
medicine
Escherichia coli
pathogenicity
Animals
Ecology, Evolution, Behavior and Systematics
Antiserum
biology
Immune Sera
DNA Viruses
biology.organism_classification
Fusion protein
Virology
Molecular biology
Specific Pathogen-Free Organisms
wsbv
Molecular Weight
Polyclonal antibodies
biology.protein
Recombinant DNA
Rabbits
baculo-like virus
penaeid shrimp
Subjects
Details
- ISSN :
- 01775103
- Volume :
- 51
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Diseases of aquatic organisms
- Accession number :
- edsair.doi.dedup.....7ad875dddb29483f9af603e5c556cbf4