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hTERT Gene Amplification and Clinical Significance in Pleural Effusions of Patients With Lung Cancer
- Source :
- Clinical Lung Cancer. 13:494-499
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Human telomerase reverse transcriptase (hTERT) gene amplification was detected in pleural effusions of patients with lung cancer (n = 69) and in patients with benign lung disease (n = 46) when using a quantitative polymerase chain reaction (qPCR) technique.hTERT gene relative copy numbers were significantly higher in effusions from patients with malignant, adenocarcinoma and small-cell lung cancer than in effusions from patients with benign lung disease (P.01). By using a threshold value of 1.39, hTERT gene amplification was significantly more frequent in malignant effusions compared with benign effusions and more likely to be positive for malignant effusions, compared with cytology (P.01). The diagnostic performance of qPCR of hTERT gene amplification was significantly higher than that of cytology, in terms of sensitivity (91.3% vs. 56.5%), negative predictive value (87.8% vs. 60.5%), and accuracy (92.2% vs. 73.9%).Detecting hTERT gene amplification by qPCR appears suitable for distinguishing carcinoma cells from reactive mesothelial cells in pleural effusions. hTERT gene amplification was more sensitive than cytology and may be useful for diagnosing pleural micrometastases.
- Subjects :
- Adult
Lung Diseases
Male
Pulmonary and Respiratory Medicine
Cancer Research
Pathology
medicine.medical_specialty
Lung Neoplasms
Pleural Neoplasms
Adenocarcinoma
Polymerase Chain Reaction
Sensitivity and Specificity
Predictive Value of Tests
Gene duplication
Biomarkers, Tumor
medicine
Carcinoma
Humans
Telomerase reverse transcriptase
Carcinoma, Small Cell
Lung cancer
Telomerase
Aged
Aged, 80 and over
business.industry
Micrometastasis
Gene Amplification
Middle Aged
respiratory system
medicine.disease
Pleural Effusion, Malignant
respiratory tract diseases
Pleural Effusion
Real-time polymerase chain reaction
Oncology
Neoplasm Micrometastasis
Cytopathology
Cancer research
Female
business
Nucleic Acid Amplification Techniques
Subjects
Details
- ISSN :
- 15257304
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Clinical Lung Cancer
- Accession number :
- edsair.doi.dedup.....7ada6805321124c274f0b94294807259