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Chemoenzyamtic synthesis and self-assembling gelation behavior of amylose-grafted poly(γ-glutamic acid)
- Source :
- International Journal of Biological Macromolecules. 97:99-105
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- In this study, we investigated chmemoenzymatic synthesis of amylose-grafted poly(γ-glutamic acid) (PGA) as a new artificial saccharide-peptide conjugate composed of two biological macromolecules. Maltooligosaccharide as a primer of enzymatic polymerization by phosphorylase catalysis was first introduced on the PGA main chain by the condensation reaction using the condensing agent in NaOH aq. Thermostable phosphorylase-catalyzed enzymatic polymerization of α-d-glucose 1-phosphate (G-1-P) as a monomer was then performed from the primer chain ends of the product to obtain amylose-grafted PGAs, which formed hydrogels in reaction media depending on the G-1-P/primer feed ratios. The powder X-ray diffraction patterns of lyophilized samples (cryogels) from the hydrogels suggested that the amylose graft chains formed double helixes, which acted as cross-inking points for self-assembling hydrogelation. The scanning electron microscopic images of the cryogels showed regularly controlled porous morphologies. Moreover, pore sizes of the cryogels increased with increasing the G-1-P/primer feed ratios, whereas the degrees of substitution of primer on the PGA main chain did not obviously affect pore sizes.
- Subjects :
- Phosphorylases
Oligosaccharides
Chemistry Techniques, Synthetic
02 engineering and technology
010402 general chemistry
01 natural sciences
Biochemistry
Polymerization
Catalysis
chemistry.chemical_compound
Structural Biology
Amylose
Polymer chemistry
Aquifoliaceae
Maltose
Molecular Biology
Chemistry
General Medicine
021001 nanoscience & nanotechnology
Condensation reaction
0104 chemical sciences
Monomer
Polyglutamic Acid
Self-healing hydrogels
Peptides
0210 nano-technology
Gels
Porosity
Macromolecule
Conjugate
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....7ae1c79226a749a3fa0ebdb331304ec7
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2017.01.001