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AKT2 interacts with Snail1 in the E-cadherin Promoter
AKT2 interacts with Snail1 in the E-cadherin Promoter
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2011
- Publisher :
- Nature Publishing Group, 2011.
-
Abstract
- 12 p.-7 fig.<br />Snail1 is a transcriptional factor essential for triggering epithelial-to-mesenchymal transition. Moreover, Snail1 promotes resistance to apoptosis, an effect associated to PTEN gene repression and Akt stimulation. In this article we demonstrate that Snail1 activates Akt at an additional level, as it directly binds to and activates this protein kinase. The interaction is observed in the nucleus and increases the intrinsic Akt activity. We determined that Akt2 is the isoform interacting with Snail1, an association that requires the pleckstrin homology domain in Akt2 and the C-terminal half in Snail1. Snail1 enhances the binding of Akt2 to the E-cadherin (CDH1) promoter and Akt2 interference prevents Snail1 repression of CDH1 gene. We also show that Snail1 binding increases Akt2 intrinsic activity on histone H3 and have identified Thr45 as a residue modified on this protein. Phosphorylation of Thr45 in histone H3 is sensitive to Snail1 and Akt2 cellular levels; moreover, Snail1 upregulates the binding of phosphoThr45 histone H3 to the CDH1 promoter. These results uncover an unexpected role of Akt2 in transcriptional control and point out to phosphorylation of Thr45 in histone H3 as a new epigenetic mark related to Snail1 and Akt2 action.Oncogene advance online publication<br />This study was supported by grants awarded by the Ministerio de Ciencia e Innovación (BFU2006-03203 and BFU2009-07578 to MD and SAF2006-00339 and SAF2010-16089 to AGH) and Fundació La Marató de TV3 (to AGH). The partial support from the Instituto Carlos III-Fondos FEDER (RTICCC, C03710, RD06/0020/0040) and the Generalitat de Catalunya (2009SGR867) is also appreciated. PV and RV-C were supported by predoctoral fellowships from the Ministerio de Ciencia y Tecnología and Instituto Carlos III, respectively
- Subjects :
- Cancer Research
Epithelial-Mesenchymal Transition
Snail1
Biology
Histones
Histone H3
Antigens, CD
Cell Line, Tumor
Genetics
Transcriptional regulation
Humans
Protein Interaction Domains and Motifs
Phosphorylation
Protein kinase A
Promoter Regions, Genetic
Molecular Biology
Protein kinase B
Psychological repression
Regulation of gene expression
AKT
EMT
E-cadherin
Cadherins
Cell biology
Fibronectins
Pleckstrin homology domain
Enzyme Activation
Gene Expression Regulation, Neoplastic
Isoenzymes
embryonic structures
Cancer research
Snail Family Transcription Factors
Protein Processing, Post-Translational
Proto-Oncogene Proteins c-akt
hormones, hormone substitutes, and hormone antagonists
Protein Binding
Transcription Factors
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Accession number :
- edsair.doi.dedup.....7ae421693eaf1f889d4ff38badb45e3e