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Neutralization of chemokines RANTES and MIG increases virus antigen expression and spinal cord pathology during Theiler's virus infection
- Source :
- International Immunology, International immunology, vol 17, iss 5
- Publication Year :
- 2005
- Publisher :
- Oxford University Press, 2005.
-
Abstract
- The role of chemokines during some viral infections is unpredictable because the inflammatory response regulated by these molecules can have two, contrasting effects—viral immunity and immunopathologic injury to host tissues. Using Theiler's virus infection of SJL mice as a model of this type of disease, we have investigated the roles of two chemokines—regulated on activation, normal T cell-expressed and secreted (RANTES) chemokine and monokine induced by IFN-γ (MIG)—by treating mice with antisera that block lymphocyte migration. Control, infected mice showed virus persistence, mild inflammation and a small degree of demyelination in the white matter of the spinal cord at 6 weeks post-infection. Treatment of mice with RANTES antiserum starting at 2 weeks post-infection increased both viral antigen expression and the severity of inflammatory demyelination at 6 weeks post-infection. MIG antiserum increased the spread of virus and the proportion of spinal cord white matter with demyelination. Overall, viral antigen levels correlated strongly with the extent of pathology. At the RNA level, high virus expression was associated with low IL-2 and high IL-10 levels, and RANTES antiserum decreased the IL-2/IL-10 ratio. Our results suggest that RANTES and MIG participate in an immune response that attempts to restrict viral expression while limiting immunopathology and that anti-chemokine treatment poses the risk of exacerbating both conditions in the long term.
- Subjects :
- Chemokine
Lymphocyte
chemokines
virus persistence
CNS central nervous system
Chemokine CXCL9
p.f.u. plaque-forming unit
Mice
0302 clinical medicine
Immunopathology
Immunology and Allergy
Viral
Lymphocytes
Antigens, Viral
Chemokine CCL5
TMEV Theiler's murine encephalomyelitis virus
0303 health sciences
IP-10 interferon-γ inducible protein-10
biology
General Medicine
DA Daniel's strain of Thelier's virus
MHV mouse hepatitis virus
3. Good health
Monokine
medicine.anatomical_structure
Spinal Cord
Cytokines
demyelination
Chemokines, CXC
Immunology
Virus
03 medical and health sciences
Immune system
Virus antigen
Theilovirus
medicine
Cardiovirus Infections
Animals
Antigens
030304 developmental biology
Antiserum
CXC
RANTES regulated on activation, normal T cell expressed and secreted
RT reverse transcriptase
Immune Sera
Virology
MIG monokine induced by IFN-γ
interleukins
biology.protein
RNA
d.p.i. days post-infection
Original Research Papers
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 14602377 and 09538178
- Volume :
- 17
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- International Immunology
- Accession number :
- edsair.doi.dedup.....7b07d3b4bd34de225faf6f28241131e9