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Mesenchymal stem/stromal cells stably transduced with an inhibitor of CC chemokine ligand 2 ameliorate bronchopulmonary dysplasia and pulmonary hypertension
- Source :
- Cytotherapy. 22:180-192
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Perinatal bronchopulmonary dysplasia (BPD) is defined as lung injury in preterm infants caused by various factors, resulting in serious respiratory dysfunction and high mortality. The administration of mesenchymal stem/stromal cells (MSCs) to treat/prevent BPD has proven to have certain therapeutic effects. However, MSCs can only weakly regulate macrophage function, which is strongly involved in the development of BPD. 7ND-MSCs are MSCs transfected with 7ND, a truncated version of CC chemokine ligand 2 (CCL2) that promotes macrophage activation, using a lentiviral vector. In the present study, we show in a BPD rat model that 7ND-MSC administration, but not MSCs alone, ameliorated the impaired alveolarization evaluated by volume density and surface area in the lung tissue, as well as pulmonary artery remodeling and pulmonary hypertension induced by BPD. In addition, 7ND-MSCs, but not MSCs alone, reduced M1 macrophages and the messenger RNA expressions of interleukin-6 and CCL2 in the lung tissue. Thus, the present study showed the treatment effect of 7ND-MSCs in a BPD rat model, which was more effective than that of MSCs alone.
- Subjects :
- Male
0301 basic medicine
Cancer Research
Stromal cell
Receptors, CCR2
Hypertension, Pulmonary
Immunology
Vascular Remodeling
CCL2
Lung injury
Mesenchymal Stem Cell Transplantation
Transfection
Viral vector
03 medical and health sciences
0302 clinical medicine
Transduction, Genetic
mental disorders
Animals
Humans
Immunology and Allergy
Medicine
Macrophage
Rats, Wistar
Chemokine CCL2
Genetics (clinical)
Bronchopulmonary Dysplasia
Transplantation
business.industry
Macrophages
Mesenchymal stem cell
Cell Biology
Macrophage Activation
medicine.disease
Pulmonary hypertension
Rats
Disease Models, Animal
HEK293 Cells
030104 developmental biology
Oncology
Bronchopulmonary dysplasia
030220 oncology & carcinogenesis
Cancer research
Female
Mutant Proteins
business
Subjects
Details
- ISSN :
- 14653249
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Cytotherapy
- Accession number :
- edsair.doi.dedup.....7b6376b333de7a0b939360923946a37b