Back to Search
Start Over
Proteomic Investigation to Identify Anticancer Targets of Nemopilema nomurai Jellyfish Venom in Human Hepatocarcinoma HepG2 Cells
- Source :
- Toxins, Vol 10, Iss 5, p 194 (2018), Toxins, Toxins; Volume 10; Issue 5; Pages: 194
- Publication Year :
- 2018
- Publisher :
- MDPI AG, 2018.
-
Abstract
- Nemopilema nomurai is a giant jellyfish that blooms in East Asian seas. Recently, N. nomurai venom (NnV) was characterized from a toxicological and pharmacological point of view. A mild dose of NnV inhibits the growth of various kinds of cancer cells, mainly hepatic cancer cells. The present study aims to identify the potential therapeutic targets and mechanism of NnV in the growth inhibition of cancer cells. Human hepatocellular carcinoma (HepG2) cells were treated with NnV, and its proteome was analyzed using two-dimensional gel electrophoresis, followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF/MS). The quantity of twenty four proteins in NnV-treated HepG2 cells varied compared to non-treated control cells. Among them, the amounts of fourteen proteins decreased and ten proteins showed elevated levels. We also found that the amounts of several cancer biomarkers and oncoproteins, which usually increase in various types of cancer cells, decreased after NnV treatment. The representative proteins included proliferating cell nuclear antigen (PCNA), glucose-regulated protein 78 (GRP78), glucose-6-phosphate dehydrogenase (G6PD), elongation factor 1γ (EF1γ), nucleolar and spindle-associated protein (NuSAP), and activator of 90 kDa heat shock protein ATPase homolog 1 (AHSA1). Western blotting also confirmed altered levels of PCNA, GRP78, and G6PD in NnV-treated HepG2 cells. In summary, the proteomic approach explains the mode of action of NnV as an anticancer agent. Further characterization of NnV may help to unveil novel therapeutic agents in cancer treatment.
- Subjects :
- 0301 basic medicine
Scyphozoa
AHSA1
Health, Toxicology and Mutagenesis
HepG2 cell
venom
lcsh:Medicine
Antineoplastic Agents
jellyfish
Nemopilema nomurai
proteomics
MALDI/TOF/MS
2-DE
Toxicology
Proteomics
Article
03 medical and health sciences
chemistry.chemical_compound
Cnidarian Venoms
Heat shock protein
Animals
Humans
Endoplasmic Reticulum Chaperone BiP
Cell Proliferation
Liver Neoplasms
lcsh:R
Hep G2 Cells
Molecular biology
Blot
030104 developmental biology
chemistry
Cancer cell
Proteome
Cancer biomarkers
Growth inhibition
Subjects
Details
- ISSN :
- 20726651
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Toxins
- Accession number :
- edsair.doi.dedup.....7b6e88801ee380fac33ea80579774a54
- Full Text :
- https://doi.org/10.3390/toxins10050194