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Direct screening of plasma circulating microRNAs
- Publication Year :
- 2018
- Publisher :
- Taylor & Francis, 2018.
-
Abstract
- Circulating microRNAs (miRNAs) are considered as reliable candidates for biomarker discovery. RNA-Sequencing has become the most suitable technique to accurately quantify the miRNAome. However, RNA-Sequencing relies on several technical passages before reaching the final-end. HTG EdgeSeq technology, thanks to the abrogation of RNA extraction step, allows productivity enhancement by reducing the number of hands-on steps, the time for sample preparation and, therefore, the costs. We found a strong correlation between qPCR and dPCR with HTG (Pearson’s coefficient of 0.93 and 0.94, respectively). In conclusion, we showed that HTG EdgeSeq, performed on human plasma specimens without RNA extraction, is reliable, allows the simultaneous screening of more than 2,000 miRNAs, and thus, it could be applied to biomarker discovery in large cohorts.
- Subjects :
- 0301 basic medicine
Sequence Analysis, RNA
RNA
Cell Biology
Computational biology
Biology
Brief Communication
03 medical and health sciences
Circulating MicroRNA
MicroRNAs
030104 developmental biology
Real-time polymerase chain reaction
microRNA
Humans
Digital polymerase chain reaction
Biomarker discovery
Molecular Biology
Biomarkers
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....7b81b6c65119831d835f5515d0ad6587