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Unexpectedly High Proportion of Ancestral Manu Genotype Mycobacterium tuberculosis Strains Cultured from Tuberculosis Patients in Egypt ▿
- Source :
- Journal of Clinical Microbiology, Journal of Clinical Microbiology, American Society for Microbiology, 2009, 47 (9), pp.2794-2801. ⟨10.1128/JCM.00360-09⟩
- Publication Year :
- 2009
- Publisher :
- American Society for Microbiology (ASM), 2009.
-
Abstract
- Tuberculosis is one of the important public health problems in Egypt. However, limited information on the Mycobacterium tuberculosis genotypes circulating in Egypt is available. A total of 151 M. tuberculosis strains were characterized by spoligotyping. The results revealed that 74.8% of M. tuberculosis isolates grouped into 13 different clusters, while 25.2% had unique spoligotype patterns. Comparison with an international spoligotyping database (the SITVIT2 database) showed that types SIT53 (T1 variant) and SIT54 (Manu2 variant) were the most common types between cluster groups. In addition, new shared types SIT2977, SIT2978, and SIT2979 were observed. The results identified for the first time an unusually high proportion of ancestral Manu strains of M. tuberculosis from patients in Egypt. The percentage of the Manu clade in this study (27.15%) was significantly higher than its overall representation of 0.4% in the SITVIT2 database. We show that in Egypt tuberculosis is caused by a predominant M. tuberculosis genotype belonging to the ancestral Manu lineage which could be a missing link in the split between ancestral and modern tubercle bacilli during the evolution of M. tuberculosis .
- Subjects :
- Male
MESH: Mycobacterium tuberculosis
"DNA Fingerprinting"
"SITVIT2"
MESH: Egypt
"Tuberculosis"
MESH: Genotype
MESH: Aged, 80 and over
Genotype
MESH: DNA Fingerprinting
Cluster Analysis
MESH: Tuberculosis
Clade
Spoligotyping
MESH: Aged
Aged, 80 and over
Molecular Epidemiology
MESH: Middle Aged
biology
"Spoligotyping"
"Manu"
Middle Aged
Bacterial Typing Techniques
DNA profiling
MESH: Young Adult
Egypt
Female
Microbiology (medical)
Adult
DNA, Bacterial
Lineage (genetic)
Tuberculosis
Adolescent
MESH: Bacterial Typing Techniques
Mycobacterium
Microbiology
Mycobacterium tuberculosis
Young Adult
"Mycobacterium"
medicine
MESH: Molecular Epidemiology
Humans
Aged
MESH: Adolescent
MESH: Humans
"Molecular Epidemiology"
Molecular epidemiology
MESH: Adult
Mycobacteriology and Aerobic Actinomycetes
biology.organism_classification
medicine.disease
MESH: Cluster Analysis
MESH: DNA, Bacterial
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Virology
DNA Fingerprinting
MESH: Male
Manu
SITVIT2
MESH: Female
Subjects
Details
- Language :
- English
- ISSN :
- 00951137
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology, Journal of Clinical Microbiology, American Society for Microbiology, 2009, 47 (9), pp.2794-2801. ⟨10.1128/JCM.00360-09⟩
- Accession number :
- edsair.doi.dedup.....7b8d86eff85d71c5324dc08b70a65f27