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Aurora B Overexpression Associates with the Thyroid Carcinoma Undifferentiated Phenotype and Is Required for Thyroid Carcinoma Cell Proliferation
- Source :
- The Journal of Clinical Endocrinology & Metabolism. 90:928-935
- Publication Year :
- 2005
- Publisher :
- The Endocrine Society, 2005.
-
Abstract
- Alterations in chromosome number (aneuploidy) are common in human neoplasias. Loss of mitotic regulation is believed to induce aneuploidy in cancer cells and act as a driving force during the malignant progression. The serine/theronine protein kinases of aurora family genes play a critical role in the regulation of key cell cycle processes. Aurora B mediates chromosome segregation by ensuring orientation of sister chromatids and overexpression of Aurora B in diploid human cells NHDF (normal human diploid fibroblast) induces multinuclearity. We analyzed Aurora B expression in human thyroid carcinomas. Cell lines originating from different histotypes showed an increase in Aurora B expression. Immunohistochemical analysis of archive samples showed a high expression of Aurora B in anaplastic thyroid carcinomas; conversely, Aurora B expression was not detectable in normal thyroid tissue. Real-time PCR analysis confirmed a strong expression of Aurora B in anaplastic thyroid carcinomas. The block of Aurora B expression induced by RNA interference or by using an inhibitor of Aurora kinase activity significantly reduced the growth of thyroid anaplastic carcinoma cells.
- Subjects :
- medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
Transplantation, Heterologous
Clinical Biochemistry
Aurora inhibitor
Aurora B kinase
Mice, Nude
Protein Serine-Threonine Kinases
Biology
Biochemistry
Gene Expression Regulation, Enzymologic
Thyroid carcinoma
Mice
Endocrinology
Aurora kinase
Aurora Kinases
Cell Line, Tumor
Internal medicine
medicine
Animals
Aurora Kinase B
Humans
Thyroid Neoplasms
Thyroid cancer
DNA Primers
Base Sequence
Reverse Transcriptase Polymerase Chain Reaction
Carcinoma
Biochemistry (medical)
Thyroid
Cell cycle
medicine.disease
Gene Expression Regulation, Neoplastic
enzymes and coenzymes (carbohydrates)
Phenotype
medicine.anatomical_structure
embryonic structures
Cancer cell
biological phenomena, cell phenomena, and immunity
Cell Division
Subjects
Details
- ISSN :
- 19457197 and 0021972X
- Volume :
- 90
- Database :
- OpenAIRE
- Journal :
- The Journal of Clinical Endocrinology & Metabolism
- Accession number :
- edsair.doi.dedup.....7ba1a31c14bb9869013f7fc05f4a3c07