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Forced Swim Alters the Radiolabeling of Blood Constituents from Wistar Rats

Authors :: Gabrielle de Souza Rocha
Carmem Santos-Fernandes
Alexandre Gonçalves de Meirelles
Nasser R Asad
Marcia de Oliveira Pereira
André L. B. D. Cardoso
Laisa Liane Paineiras-Domingos
Marcia Cristina Moura-Fernandes
Marco Antonio de Souza Gama
Adenilson de Souza da Fonseca
Danúbia da Cunha de Sá-Caputo
Mario Bernardo-Filho
Daniel Batouli-Santos
Eric Heleno Freire Ferreira Frederico
Redha Taiar
Tiago Eduardo-Santos
Source :: Applied Sciences, Volume 10, Issue 3, Applied Sciences, Vol 10, Iss 3, p 1116 (2020)
Publication Year :: 2020
Publisher :: Multidisciplinary Digital Publishing Institute, 2020.
Abstract: The present study investigated the effects of forced swimming on the technetium-99m (99mTc) labeling of blood constituents (BloCs). Rats (Wistar) were submitted to forced swim. In previous experiments, swimming animals would recover for different periods of time. Animals not submitted to swimming were used as control. Blood samples were obtained and the 99mTc labeling of BloCs was carried out. Blood cells (BCs), plasma (P), insoluble fractions (IF-P and IF-BCs), and soluble fractions (SF-P and SF-BC) were isolated. Radioactivity was determined, and the percentage of 99mTc incorporated (%ATI) was calculated in each fraction. Results showed that forced swimming decreased the percentage of 99mTc incorporated (%ATI) in IF-P (p &lt<br />0.05). It is suggested that the 99mTc labeling of BloCs could be used to verify the effects of the stress conditions on BloCs and that the radionuclide fixation on plasma proteins might be altered in rats submitted to acute stress induced by forced swimming, returning to control levels after recovery.