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Molecular evaluation of chronic restrain stress in mice model of non metastatic fibrosarcoma

Authors :
Anca Maria Cimpean
Pavle Banović
Verica Simin
Dusan Lalosevic
Gordana Smieško
Vera Gusman
Source :
Journal of Molecular Histology. 51:367-374
Publication Year :
2020
Publisher :
Springer Science and Business Media LLC, 2020.

Abstract

Chronic stress is regarded as a significant factor in the etiology of the many diseases. Numerous methods have been developed through which the effect of chronic stress is examined. The aim of this study is to demonstrate the new experimental model for analysis of immuno-suppression induced by chronic restraint stress, through challenge with conditionally tumorigenic cell line BHK-21/C13. 20 male NMRI mice were randomly divided into 2 groups-control and experimental. Each mouse was subcutaneously inoculated with BHK-21/C13 cells. Stress in the experimental group was induced for 20 days. After the experiment, tumor masses were removed, and analyzed using histology and immunohistochemistry techniques. We found a statistically significant difference (p = 0.034) in tumor expression and tumor volumes (p = 0.0061) between groups, as well as in immunopositivity on Ki67, cytochrome C and matrix metalloproteinase 9. Absence of immune infiltrate was noticed in experimental, and the presence of inflammatory infiltrate at tumor invasion front in control group.

Details

ISSN :
15672387 and 15672379
Volume :
51
Database :
OpenAIRE
Journal :
Journal of Molecular Histology
Accession number :
edsair.doi.dedup.....7c18c71d69ba652d22ffc0d5d87e3a17
Full Text :
https://doi.org/10.1007/s10735-020-09886-5