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Modulation of Chromatin Remodelling Induced by the Freshwater Cyanotoxin Cylindrospermopsin in Human Intestinal Caco-2 Cells
- Source :
- PLoS ONE, PLoS ONE, Public Library of Science, 2014, 9 (6), pp.e99121. ⟨10.1371/journal.pone.0099121⟩, PLoS ONE, Vol 9, Iss 6, p e99121 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- International audience; Cylindrospermopsin (CYN) is a cyanotoxin that has been recognised as an emerging potential public health risk. Although CYN toxicity has been demonstrated, the mechanisms involved have not been fully characterised. To identify some key pathways related to this toxicity, we studied the transcriptomic profile of human intestinal Caco-2 cells exposed to a sub-toxic concentration of CYN (1.6 mM for 24hrs) using a non-targeted approach. CYN was shown to modulate different biological functions which were related to growth arrest (with down-regulation of cdkn1a and uhrf1 genes), and DNA recombination and repair (with up-regulation of aptx and pms2 genes). Our main results reported an increased expression of some histone-modifying enzymes (histone acetyl and methyltransferases MYST1, KAT5 and EHMT2) involved in chromatin remodelling, which is essential for initiating transcription. We also detected greater levels of acetylated histone H2A (Lys5) and dimethylated histone H3 (Lys4), two products of these enzymes. In conclusion, CYN overexpressed proteins involved in DNA damage repair and transcription, including modifications of nucleosomal histones. Our results highlighted some new cell processes induced by CYN.
- Subjects :
- Methyltransferase
Cellular differentiation
lcsh:Medicine
Toxicology
chemistry.chemical_compound
Histocompatibility Antigens
cylindrospermopsin
toxicité
Toxins
lcsh:Science
Histone Acetyltransferases
Lysine Acetyltransferase 5
Multidisciplinary
Cyanobacteria Toxins
biology
cyanotoxin
Genomics
caco-2: freshwater
3. Good health
Cell biology
Histone
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Transcriptome Analysis
Research Article
Cyclin-Dependent Kinase Inhibitor p21
human intestinal caco-2 cell
cyanotoxine
DNA repair
Ubiquitin-Protein Ligases
Bacterial Toxins
Toxic Agents
Down-Regulation
Histone H3
Alkaloids
Genetics
Humans
Uracil
KAT5
Toxicity
lcsh:R
toxicologie
Biology and Life Sciences
Computational Biology
Histone-Lysine N-Methyltransferase
Chromatin Assembly and Disassembly
Genome Analysis
Molecular biology
Enterocytes
chemistry
cylindrospermopsine
CCAAT-Enhancer-Binding Proteins
biology.protein
lcsh:Q
Cylindrospermopsin
Caco-2 Cells
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....7c434d594fcb16fe49b8c800ad0945da
- Full Text :
- https://doi.org/10.1371/journal.pone.0099121