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Fungal effector SIB1 of Colletotrichum orbiculare has unique structural features and can suppress plant immunity in Nicotiana benthamiana
- Source :
- The Journal of Biological Chemistry
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Fungal plant pathogens secrete virulence-related proteins, called effectors, to establish host infection; however, the details are not fully understood yet. Functional screening of effector candidates using Agrobacterium-mediated transient expression assay in Nicotiana benthamiana identified two virulence-related effectors, named SIB1 and SIB2 (Suppression of Immunity in N. benthamiana), of an anthracnose fungus Colletotrichum orbiculare, which infects both cucurbits and N. benthamiana. The Agrobacterium-mediated transient expression of SIB1 or SIB2 increased the susceptibility of N. benthamiana to C. orbiculare, which suggested these effectors can suppress immune responses in N. benthamiana. The presence of SIB1 and SIB2 homologs was found to be limited to the genus Colletotrichum. SIB1 suppressed both (i) the generation of reactive oxygen species triggered by two different pathogen-associated molecular patterns, chitin and flg22, and (ii) the cell death response triggered by the Phytophthora infestans INF1 elicitin in N. benthamiana. We determined the NMR-based structure of SIB1 to obtain its structural insights. The three-dimensional structure of SIB1 comprises five β-strands, each containing three disulfide bonds. The overall conformation was found to be a cylindrical shape, such as the well-known antiparallel β-barrel structure. However, the β-strands were found to display a unique topology, one pair of these β-strands formed a parallel β-sheet. These results suggest that the effector SIB1 present in Colletotrichum fungi has unique structural features and can suppress pathogen-associated molecular pattern–triggered immunity in N. benthamiana.
- Subjects :
- Protein Conformation
Colletotrichum orbiculare
Agrobacterium
Nicotiana benthamiana
Biochemistry
susceptibility
PCD, programmed cell death
HR, hypersensitive response
Virulence
biology
Effector
food and beverages
PDA, potato dextrose agar
Cell biology
three-dimensional structure
effector
Host-Pathogen Interactions
RBOH, respiratory burst oxidase homolog
plant immunity
PAMP, pathogen-associated molecular pattern
Research Article
Hypersensitive response
hpi, hours post inoculation
ToMV, tobamovirus
dpi, days post inoculation
Plant Immunity
PGAP, pyroglutamate aminopeptidase
PTI, pathogen-associated molecular pattern–triggered immunity
Fungal Proteins
ROS, reactive oxygen species
PDB, Protein Data Bank
Tobacco
Colletotrichum
Amino Acid Sequence
eGFP, enhanced GFP
Molecular Biology
Sequence Homology, Amino Acid
Pathogen-associated molecular pattern
TEAB, triethylamine bicarbonate
fungi
Elicitin
Cell Biology
biology.organism_classification
BY-2, Nicotiana tabacum cv. Bright Yellow 2
qPCR, quantitative PCR
Reactive Oxygen Species
IAA, iodoacetamide
SIB, Suppression of Immunity in N.benthamiana
Subjects
Details
- Language :
- English
- ISSN :
- 00219258
- Volume :
- 297
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....7c6dc53745496cd59eb53d68220bf094