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Alteration of cytochrome P450 1 regulation and HSP 70 level in brain of juvenile common carp (Cyprinus carpio) after chronic exposure to tributyltin
- Source :
- Fish physiology and biochemistry. 42(1)
- Publication Year :
- 2015
-
Abstract
- Tributyltin (TBT), a toxic contaminant in aquatic environments, has bio-accumulated in aquatic food webs throughout the world and can be found at toxic levels in some biota. However, the molecular mechanisms and effects of TBT are not fully understood. The aim of the present study was to investigate the effect of long-term exposure of TBT on cytochrome P450 (CYP450) 1 regulation and heat-shock proteins (HSPs) profiling in brain of freshwater teleost. The effects of long-term exposure to TBT on mRNA expression of cytochrome P450 (CYP450) 1 family genes and ethoxyresorufin O-deethylase (EROD) activity in the brain of common carp were evaluated, as well as HSP 70 level. Fish were exposed to sublethal concentrations of TBT (75 ng/L, 0.75 μg/L and 7.5 μg/L) for 15, 30, and 60 days. Based on the results, long-term exposure (more than 15 days) to TBT could lead to obvious physiological–biochemical responses (based on EROD activity, HSP 70 level and CYP450 1 family genes expression). The mRNA expression of CYP450 1 family genes (CYP1A, CYP1B, CYP1C1 and CYP1C2) suggested that CYP1A was to accommodate most EROD activity in fish, but other CYP450 forms also involved in this proceeding. Thus, the measured physiological responses in fish brain could provide useful information to better understand the mechanisms of TBT-induced bio-toxicity and could be used as potential biomarkers for monitoring the TBT pollution in the field.
- Subjects :
- 0301 basic medicine
Chronic exposure
Carps
Physiology
Gene Expression
010501 environmental sciences
Aquatic Science
01 natural sciences
Biochemistry
Cyprinus
03 medical and health sciences
Common carp
chemistry.chemical_compound
Cytochrome P-450 Enzyme System
Heat shock protein
Juvenile
Animals
HSP70 Heat-Shock Proteins
RNA, Messenger
Gene
0105 earth and related environmental sciences
biology
Ecology
Cytochrome P450
Brain
General Medicine
biology.organism_classification
030104 developmental biology
chemistry
biology.protein
Tributyltin
Trialkyltin Compounds
Water Pollutants, Chemical
Subjects
Details
- ISSN :
- 15735168
- Volume :
- 42
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Fish physiology and biochemistry
- Accession number :
- edsair.doi.dedup.....7c7be98067103287853a0c8172894cd8