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Heterogeneous nuclear ribonuclear protein K interacts with the enterovirus 71 5′ untranslated region and participates in virus replication
- Source :
- Journal of General Virology. 89:2540-2549
- Publication Year :
- 2008
- Publisher :
- Microbiology Society, 2008.
-
Abstract
- Enterovirus 71 (EV71) is a picornavirus that can cause severe neurological complications in children. Like other picornaviruses, the genomic RNA of EV71 contains a long 5′ untranslated region (UTR). Cellular proteins interact with the EV71 5′ UTR, and these interactions are important for virus replication. Using an RNA pull-down assay and proteomics approaches, this study identified the heterogeneous nuclear ribonucleoprotein K (hnRNP K) as one of the EV71 5′ UTR-associated proteins. The interaction between hnRNP K and the 5′ UTR was further confirmed by mapping the interaction regions to stem–loops I–II and IV in the 5′ UTR. During EV71 infection, hnRNP K was enriched in the cytoplasm where virus replication occurs, whereas hnRNP K was localized in the nucleus in mock-infected cells. Viral yields were found to be significantly lower in hnRNP K knockdown cells and viral RNA synthesis was delayed in hnRNP K knockdown cells in comparison with negative-control cells treated with small interfering RNA. These results suggest that hnRNP K interacts with the EV71 5′ UTR and participates in virus replication.
- Subjects :
- Untranslated region
Cytoplasm
Small interfering RNA
Five prime untranslated region
Picornavirus
biology
viruses
Protein K (gene expression)
RNA
Virus Replication
biology.organism_classification
environment and public health
Virology
Molecular biology
Heterogeneous-Nuclear Ribonucleoprotein K
Viral replication
Cell Line, Tumor
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
biology.protein
Humans
RNA, Viral
5' Untranslated Regions
Enterovirus
Subjects
Details
- ISSN :
- 14652099 and 00221317
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Journal of General Virology
- Accession number :
- edsair.doi.dedup.....7c80acadedb2cb1355ba11b392fa6b19