Interfacing with patterned in vitro neural networks by means of hybrid glass-elastomer neurovectors: Progress on neuron placement, neurite outgrowth and biopotential measurements

In order to extract learning algorithms from living neural aggregates it would be advantageous to achieve one-to-one neuron-electrode interfacing with in vitro networks. Towards this goal, we have developed a hybrid glass-elastomer technology, which allows topology specification in small networks (of the order of 10 neurons) and recording of extracellular potentials from individual neurites grown through microfluidic channels. Here we report on progress towards adhesion-free placement of cells within microwells, promotion of neurite growth and recording of intra-channel extracellular spikes.