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Chicken lutropin acts like follitropin in rat ovarian follitropin receptor: An isoelectric focusing study
- Source :
- Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology. 120:83-89
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- This study investigates whether chicken lutropin (LH) specifically binds to rat ovarian follitropin (FSH) receptor and exerts FSH-like bioactivity. Glycoprotein fraction, prepared from the chicken anterior pituitary gland, was fractionated using isoelectric focusing within a pH range of 3.5-11. Analysis of the focused fractions, by a radioreceptor assay (RRA) specific for FSH in rats using rat ovarian homogenate as receptor source, and 125I-labeled rat FSH as radioligand, detected a large component having an isoelectric point of 10.25. This focusing profile obtained by RRA was quite similar to that obtained by a specific radioimmunoassay (RIA) for chicken LH, but clearly different from that obtained by a specific RIA for chicken FSH, indicating this RRA specifically recognizes chicken LH. Chicken LH fraction prepared from the electrofocused material was used for further studies. The chicken LH preparation was three times more potent than rat FSH in the RRA in displacing the radioligand bound to rat ovarian receptor, while chicken LH facilitated an 8-fold less production of estradiol in dispersed rat granulosa cells than rat FSH. These results suggest that chicken LH acts like rat FSH in rat ovarian FSH receptor, but receptor-binding activity is much higher than biological activity.
- Subjects :
- Male
endocrine system
medicine.medical_specialty
animal structures
Immunology
Radioimmunoassay
Follitropin
Biology
Iodine Radioisotopes
Species Specificity
Anterior pituitary
Pituitary Gland, Anterior
Internal medicine
medicine
Radioligand
Animals
Rats, Wistar
Receptor
Pharmacology
Chromatography
Isoelectric focusing
Sepharose
Ovary
Luteinizing Hormone
Rats
Endocrinology
medicine.anatomical_structure
Isoelectric point
Receptors, FSH
Female
Follicle Stimulating Hormone
Isoelectric Focusing
Follicle-stimulating hormone receptor
Chickens
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 07428413
- Volume :
- 120
- Database :
- OpenAIRE
- Journal :
- Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology
- Accession number :
- edsair.doi.dedup.....7ccb579844da30e9d0a5cd3de3d60a9c