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Effects of histone acetylation on chromatin structure
- Source :
- Journal of Cellular Biochemistry. 64:466-475
- Publication Year :
- 1997
- Publisher :
- Wiley, 1997.
-
Abstract
- The effect of histone acetylation was monitored on CHO chromatin structure, following the addition of 7 mM Na-butyrate to the cell culture medium. The properties of both control and hyperacetylated chromatins and nuclei were investigated by circular dichroism, ethidium bromide intercalation, differential scanning calorimetry, and affinity chromatography. Our results are compatible with modest but significant alterations in the various levels of chromatin organization, as a result of the charge neutralization of some lysine residues within the N-terminal region of the histonic octamer. Namely, large statistically significant differences do exist in the heat capacity thermograms of native nuclei, where unfolding into single nucleofilament of the highly packed native chromatin superfiber appears associated with acetylation; at the same time CD, EB, and affinity chromatography point to modest but consistent differences in the compactness of isolated nucleosomes and polynucleosomes.
- Subjects :
- Circular dichroism
CHO Cells
Calorimetry
Biochemistry
Chromatography, Affinity
Histones
chemistry.chemical_compound
Affinity chromatography
Cricetinae
Animals
Nucleosome
Histone octamer
Molecular Biology
biology
Circular Dichroism
Temperature
Acetylation
Cell Biology
Chromatin
Histone
chemistry
biology.protein
Ethidium bromide
Subjects
Details
- ISSN :
- 10974644 and 07302312
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....7ccd2757c0dd3276bf836918ed4faec9