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The wing of the ToxR winged helix-turn-helix domain is required for DNA binding and activation of toxT and ompU

Authors :
Sarah J. Morgan
Emily L. French
Sarah C. Plecha
Eric S. Krukonis
Source :
PLoS ONE, Vol 14, Iss 9, p e0221936 (2019), PLoS ONE
Publication Year :
2019
Publisher :
Public Library of Science (PLoS), 2019.

Abstract

ToxR and TcpP, two winged helix-turn-helix (w-HTH) family transcription factors, co-activate expression of the toxT promoter in Vibrio cholerae. ToxT then directly regulates a number of genes required for virulence. In addition to co-activation of toxT, ToxR can directly activate the ompU promoter and repress the ompT promoter. Based on a previous study suggesting that certain wing residues of ToxR are preferentially involved in toxT co-activation compared to direct ompU activation, we employed alanine-scanning mutagenesis to determine which residues in the wing of ToxR are required for activation of each promoter. All of the ToxR wing residues tested that were critical for transcriptional activation of toxT and/or ompU were also critical for DNA binding. While some ToxR wing mutants had reduced interaction with TcpP, that reduced interaction did not correlate with a specific defect in toxT activation. Rather, such mutants also affected ompU activation and DNA binding. Based on these findings we conclude that the primary role of the wing of ToxR is to bind DNA, along with the DNA recognition helix of ToxR, and this function is required both for direct activation of ompU and co-activation of toxT.

Details

Language :
English
ISSN :
19326203
Volume :
14
Issue :
9
Database :
OpenAIRE
Journal :
PLoS ONE
Accession number :
edsair.doi.dedup.....7ce4fc7794b7e071cd1b6583b77b0929