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Scientific Reports
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Nature Publishing Group, 2016.
-
Abstract
- Recent studies showed that GPR30, a seven-transmembrane G-protein-coupled receptor, is a novel estrogen receptor (ER) that mediates some biological events elicited by estrogen in several types of cancer cells. However, its physiological or pathological role in vivo is unclear. Here, we show that GPR30 knockout (GPRKO) female mice were protected from high-fat diet (HFD)-induced obesity, blood glucose intolerance and insulin resistance. The decreased body weight gain in GPRKO female mice is due to the reduction in body fat mass. These effects occurred in the absence of significant changes in food intake, intestinal fat absorption, triglyceride metabolism, or energy expenditure. However, GPR30 had no significant metabolic effects in male mice fed the HFD and both sexes of mice fed a chow diet. Further, GPR30 expression levels in fat tissues of WT obese female mice were greatly increased, whereas ERα and β expression was not altered. Deletion of GPR30 reduced adipogenic differentiation of adipose tissue-derived stromal cells. Conversely, activation of GPR30 enhanced adipogenic differentiation of 3T3-L1 preadipocytes. These findings provide evidence for the first time that GPR30 promotes adipogenesis and therefore the development of obesity in female mice exposed to excess fat energy.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
medicine.drug_class
Adipose tissue
Estrogen receptor
030209 endocrinology & metabolism
fatty-acid oxidation
g-protein-coupled-receptor-30 gpr30
In Vitro Techniques
Biology
Diet, High-Fat
Article
Receptors, G-Protein-Coupled
insulin-resistance
Mice
g-protein
03 medical and health sciences
0302 clinical medicine
Insulin resistance
coupled receptor 30
Internal medicine
medicine
Animals
up-regulation
Receptor
Adiposity
estrogen-receptor
2. Zero hunger
Adipogenesis
Multidisciplinary
Estradiol
breast-cancer cells
Fatty Acids
energy-expenditure
plasma-membrane
medicine.disease
Obesity
030104 developmental biology
Endocrinology
Receptors, Estrogen
Estrogen
Female
Energy Metabolism
Oxidation-Reduction
GPER
Body Temperature Regulation
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....7cec3f998ad4aae5e48f4153c4095458
- Full Text :
- https://doi.org/10.1038/srep34302