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Cellular Models and Assays to Study NLRP3 Inflammasome Biology
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 21, Iss 4294, p 4294 (2020)
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- The NLRP3 inflammasome is a multi-protein complex that initiates innate immunity responses when exposed to a wide range of stimuli, including pathogen-associated molecular patterns (PAMPs) and danger-associated molecular patterns (DAMPs). Inflammasome activation leads to the release of the pro-inflammatory cytokines interleukin (IL)-1β and IL-18 and to pyroptotic cell death. Over-activation of NLRP3 inflammasome has been associated with several chronic inflammatory diseases. A deep knowledge of NLRP3 inflammasome biology is required to better exploit its potential as therapeutic target and for the development of new selective drugs. To this purpose, in the past few years, several tools have been developed for the biological characterization of the multimeric inflammasome complex, the identification of the upstream signaling cascade leading to inflammasome activation, and the downstream effects triggered by NLRP3 activation. In this review, we will report cellular models and cellular, biochemical, and biophysical assays that are currently available for studying inflammasome biology. A special focus will be on those models/assays that have been used to identify NLRP3 inhibitors and their mechanism of action.
- Subjects :
- Programmed cell death
2019-20 coronavirus outbreak
Inflammasomes
Interleukin-1beta
Review
Biology
Biochemical assays
Models, Biological
Catalysis
Inflammasome
lcsh:Chemistry
Inorganic Chemistry
NLRP3
NLR Family, Pyrin Domain-Containing 3 Protein
Pyroptosis
medicine
Deep knowledge
Alarmins
Animals
Humans
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Innate immune system
integumentary system
Cell models
Pathogen-Associated Molecular Pattern Molecules
Organic Chemistry
Interleukin-18
Interleukin
General Medicine
Biophysical assays
Immunity, Innate
Computer Science Applications
Cell biology
NLRP3 inhibitors
lcsh:Biology (General)
lcsh:QD1-999
Mechanism of action
Read-outs
medicine.symptom
Inflammasome complex
Signal Transduction
medicine.drug
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....7d18641f14dd8bd949b1cfc31ad5e6d2