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Sexual disruption in zebrafish ( Danio rerio ) exposed to mixtures of 17α-ethinylestradiol and 17β-trenbolone
- Source :
- Örn, S, Holbech, H & Norrgren, L 2016, ' Sexual disruption in zebrafish ( Danio rerio ) exposed to mixtures of 17α-ethinylestradiol and 17β-trenbolone ', Environmental Toxicology and Pharmacology, vol. 41, no. January, pp. 225-231 . https://doi.org/10.1016/j.etap.2015.12.010
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Environmental estrogens and androgens can be present simultaneously in aquatic environments and thereby interact to disturb multiple physiological systems in organisms. Studies on interaction effects in fish of androgenic and estrogenic chemicals are limited. Therefore, the aim of the present study was to evaluate feminization and masculinization effects in zebrafish (Danio rerio) exposed to combinations of two synthetic steroid hormones detected in environmental waters: the androgen 17β-trenbolone (Tb) and the oestrogen 17α-ethinylestradiol (EE2). Juvenile zebrafish were exposed between days 20 and 60 post-hatch to different binary mixtures of Tb (1, 10, and 50 ng/L) and EE2 (2 and 5 ng/L). The endpoints studied were whole-body homogenate vitellogenin concentration at 40 days post-hatch, and sex ratio including gonad maturation at 60 days post-hatch. The feminizing potency of 5 ng/L of EE2, alone as well as in combination with Tb, was clear in the present study, with exposures resulting in almost all-female populations and females being sexually immature. Masculinization effects with male-biased sex ratios were observed when fish were exposed to 2 ng/L of EE2 in combination with Tb concentrations. Intersex fish were observed after exposure to mixtures of 2 ng/L EE2 with 50 ng/L Tb. Sexual maturity generally increased among males at increasing concentrations of Tb. The results of the present study show that exposure to environmentally relevant mixtures of an oestrogen and androgen affects the process of gonad differentiation in zebrafish and lead to sexual disruption.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Sex Differentiation
Gonad
medicine.drug_class
Health, Toxicology and Mutagenesis
Feminization (biology)
17α-Ethinylestradiol
Danio
010501 environmental sciences
Ethinyl Estradiol
Toxicology
17β-Trenbolone
01 natural sciences
Vitellogenins
03 medical and health sciences
Vitellogenin
Trenbolone
Internal medicine
medicine
Animals
Sexual maturity
Sexual Maturation
Gonads
Zebrafish
0105 earth and related environmental sciences
Pharmacology
Sex Characteristics
biology
General Medicine
sex ratio
Androgen
biology.organism_classification
030104 developmental biology
medicine.anatomical_structure
Endocrinology
biology.protein
Female
Trenbolone Acetate
vitellogenin
Water Pollutants, Chemical
medicine.drug
Subjects
Details
- ISSN :
- 13826689
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Environmental Toxicology and Pharmacology
- Accession number :
- edsair.doi.dedup.....7d30a8349003f5189ce7269ccb7b98d1