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TEEG Induced A549 Cell Autophagy by Regulating the PI3K/AKT/mTOR Signaling Pathway
- Source :
- Analytical Cellular Pathology, Vol 2019 (2019), Analytical Cellular Pathology (Amsterdam)
- Publication Year :
- 2019
- Publisher :
- Hindawi Limited, 2019.
-
Abstract
- TEEG (3β,16β,23-trihydroxy-13,28-epoxyurs-11-ene-3-O-β-D-glucopyranoside) is derived from the chloroform extract of the Chinese medicine formula Shenqi San (CE-SS). In the present study, we aimed to elucidate the anticancer effect and possible molecular mechanism underlying the action of TEEG against the human non-small cell lung cancer (NSCLC) cell line A549 in vitro. A549 cells were incubated with different concentrations of TEEG. Cell proliferation was assessed by MTT assay. Autophagy was evaluated by immunofluorescence staining. Autophagy-associated proteins were examined by Western blot analysis. TEEG markedly inhibited A549 cell proliferation in a concentration-dependent manner. Immunofluorescence staining showed that TEEG induced autophagy in A549 cells. The LC3-II : LC3-I conversion ratio and the expression of Beclin-1, Atg5, Atg7, and Atg12 increased with the concentration of TEEG. In addition, increased TEEG concentration enhanced the expression of Class III p-PI3K and reduced the expression of Class I p-PI3K, p-AKT, p-mTOR, and p-P70S6K. These results indicate that TEEG induces autophagy of A549 cells through regulation of the PI3K/AKT/mTOR signaling pathway.
- Subjects :
- 0301 basic medicine
Cancer Research
Lung Neoplasms
Article Subject
ATG5
Antineoplastic Agents
Pathology and Forensic Medicine
ATG12
03 medical and health sciences
Phosphatidylinositol 3-Kinases
0302 clinical medicine
Glucosides
Carcinoma, Non-Small-Cell Lung
Autophagy
Humans
MTT assay
Protein kinase B
PI3K/AKT/mTOR pathway
RC254-282
Cell Proliferation
A549 cell
QH573-671
Chemistry
Cell growth
TOR Serine-Threonine Kinases
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Cell Biology
General Medicine
Cell biology
030104 developmental biology
A549 Cells
030220 oncology & carcinogenesis
Molecular Medicine
Cytology
Proto-Oncogene Proteins c-akt
Drugs, Chinese Herbal
Signal Transduction
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 22107185 and 22107177
- Volume :
- 2019
- Database :
- OpenAIRE
- Journal :
- Analytical Cellular Pathology
- Accession number :
- edsair.doi.dedup.....7d49cf68fe96e03402dfa9f71fec0d8a