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T cell reactivity to P0, P2, PMP-22, and myelin basic protein in patients with Guillain-Barre syndrome and chronic inflammatory demyelinating polyradiculoneuropathy
- Source :
- Journal of neurology, neurosurgery, and psychiatry. 76(10)
- Publication Year :
- 2005
-
Abstract
- Objectives: It has been suggested that autoimmunity to peripheral myelin proteins is involved in the pathogenesis of Guillain-Barre syndrome (GBS) and chronic inflammatory demyelinating polyradiculoneuropathy (CIDP). We aimed to compare reactivity of peripheral blood mononuclear cells (PBMC) to antigens of peripheral myelin proteins in patients with GBS and patients with CIDP with that of healthy controls and patients with other non-immune mediated neuropathies (ON). Methods: We prepared PBMC from blood from 83 healthy controls and from 64 patients with GBS, 54 with CIDP, and 62 with ON. PBMC were tested in antigen specific proliferation assays against peptides from myelin proteins P0, P2, PMP22, and myelin basic protein (MBP), which is identical to myelin P1, and against whole human MBP. Interferon-gamma (IFN-γ) and interleukin (IL)-5 enzyme linked immunospot (ELISPOT) assays were also performed in some subjects to assess spontaneous and peripheral myelin antigen specific PBMC cytokine secretion. Results: Antigen specific PBMC proliferation assays showed no significant elevation of peptide specific T cell responsiveness in patients with GBS or CIDP compared with healthy controls or patients with ON. Levels of spontaneous ELISPOT IFN-γ secretion were increased in patients with GBS and significantly increased in those with CIDP compared with healthy controls and patients with ON. No convincing differences in antigen specific ELISPOT IFN-γ secretion levels to individual peptides were detectable in patients with GBS. The proportion of patients with CIDP with an increased number of PBMC producing IFN-γ in response to peptide PMP-22 51–64 was significantly increased compared with healthy controls and patients with ON. No significant differences in antigen specific ELISPOT IL-5 secretion levels were detectable in patients with GBS or CIDP compared with controls, but levels of spontaneous IL-5 secretion were significantly higher in patients with CIDP than in healthy controls or patients with ON. Conclusions: Although the lack of significantly increased antigen specific PBMC proliferation in GBS and CIDP does not support a role for T cells, the more sensitive ELISPOT technique detected increased numbers of PBMC secreting IFN-γ spontaneously in 25% of patients with GBS, providing further evidence for a role of T cells in the immunopathology of GBS. Increased numbers of spontaneous IFN-γ and IL-5 secreting cells, and increased IFN-γ secretion in response to PMP-22 51–64 , in patients with CIDP provide further evidence for a role of myelin specific T cells in CIDP.
- Subjects :
- Adult
Male
Paper
medicine.medical_specialty
Adolescent
T cell
T-Lymphocytes
Guillain-Barre Syndrome
Myelin P2 Protein
Peripheral blood mononuclear cell
Myelin
Interferon-gamma
Th2 Cells
Antigen
Internal medicine
medicine
Humans
Aged
Aged, 80 and over
biology
business.industry
Multiple sclerosis
ELISPOT
Myelin Basic Protein
Middle Aged
Th1 Cells
medicine.disease
Myelin basic protein
Psychiatry and Mental health
Endocrinology
medicine.anatomical_structure
Polyradiculoneuropathy, Chronic Inflammatory Demyelinating
Immunology
biology.protein
bacteria
Cytokines
Surgery
Cytokine secretion
Female
Neurology (clinical)
Interleukin-5
business
Myelin P0 Protein
Myelin Proteins
Subjects
Details
- ISSN :
- 00223050
- Volume :
- 76
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of neurology, neurosurgery, and psychiatry
- Accession number :
- edsair.doi.dedup.....7d6f2944d1601b6f4b1c8fec947f433f