Back to Search
Start Over
Zoosystematics and Evolution
- Source :
- CIÊNCIAVITAE, Zoosystematics and Evolution 96(1): 175-193, Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos), Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação, instacron:RCAAP, Zoosystematics and Evolution, Vol 96, Iss 1, Pp 175-193 (2020)
- Publication Year :
- 2020
-
Abstract
- The genus Longidorus currently comprises 176 species of polyphagous plant ectoparasites, including eight species that vector nepo-viruses. Longidorus is one of the most difficult genera to accurately identify species because of the similar morphology and overlapping measurements and ratios among species. Sequences of ribosomal RNA (rRNA)-genes are a powerful level-species diagnostic tool for the genus Longidorus. From 2015 to 2019, a nematode survey was conducted in vineyards and agro-forest environments in Portugal. The populations of Longidorus spp. were characterized through an integrative approach based on morphological data and molecular phylogenetic analysis from rRNA genes (D2-D3 expansion segments of the 28S, ITS1, and partial 18S), including the topotype of L. vinearum. Longidorus bordonensis sp. nov., a didelphic species recovered from the rhizosphere of grasses, is described and illustrated. Longidorus vineacola, with cork oak and wild olive as hosts, is also characterized. This is the first time that L. wicuolea, from cork oak, is reported for Portugal. Bayesian inference (BI) phylogenetic trees for these three molecular markers established phylogenetic relationships among the new species with other Longidorus spp. Phylogenetic trees indicated that i) L. bordonensis sp. nov. is clustered together with other Longidorus spp. and forms a sister clade with L. pini and L. carpetanensis, sharing a short body and odontostyle length, and elongate to conical female tail, and ii) all the other species described and illustrated are phylogenetically associated, including the topotype isolate of L. vinearum. Foundation for Science and Technology (Portugal)Portuguese Foundation for Science and Technology [UID/AGR/00115/2019] This research was financially supported by National Funds through Foundation for Science and Technology under the project UID/AGR/00115/2019 (Portugal). We thank M.I. Ferreira and G. Albarran from Instituto de Ciencias Agrarias e Ambientais Mediterranicas, Universidade of Evora for their excellent technical assistance.
- Subjects :
- 0106 biological sciences
Systematics
D2-D3 expansion segments of large ribosomal subunit 28S
Nematoda
needle nematodes
Genomic data
Dorylaimida
Bayesian inference
010607 zoology
Bayesian inference D2–D3 expansion segments of large ribosomal subunit 28S genomic data needle nematodes internal transcribed spacer 1 partial small ribosomal subunit
Adenophorea
01 natural sciences
partial small ribosomal subunit
Genus
Animalia
Longidorus
Longidoridae
D2–D3 expansion segments of large ribosomal subunit 28S
internal transcribed spacer 1
lcsh:QH301-705.5
Ecology, Evolution, Behavior and Systematics
biology
biology.organism_classification
language.human_language
Geography
lcsh:Biology (General)
Evolutionary biology
Molecular phylogenetics
language
genomic data
Portuguese
010606 plant biology & botany
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- CIÊNCIAVITAE, Zoosystematics and Evolution 96(1): 175-193, Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos), Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação, instacron:RCAAP, Zoosystematics and Evolution, Vol 96, Iss 1, Pp 175-193 (2020)
- Accession number :
- edsair.doi.dedup.....7d70a863652f2971f0bf48fca4306997