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Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation
- Source :
- BMC Microbiology, Vol 11, Iss 1, p 146 (2011), BMC Microbiology, BMC Microbiology, Vol. 11 (2011) P. 146
- Publisher :
- Springer Nature
-
Abstract
- Background Staphylococcus epidermidis (SE) has emerged as one of the most important causes of nosocomial infections. The SaeRS two-component signal transduction system (TCS) influences virulence and biofilm formation in Staphylococcus aureus. The deletion of saeR in S. epidermidis results in impaired anaerobic growth and decreased nitrate utilization. However, the regulatory function of SaeRS on biofilm formation and autolysis in S. epidermidis remains unclear. Results The saeRS genes of SE1457 were deleted by homologous recombination. The saeRS deletion mutant, SE1457ΔsaeRS, exhibited increased biofilm formation that was disturbed more severely (a 4-fold reduction) by DNase I treatment compared to SE1457 and the complementation strain SE1457saec. Compared to SE1457 and SE1457saec, SE1457ΔsaeRS showed increased Triton X-100-induced autolysis (approximately 3-fold) and decreased cell viability in planktonic/biofilm states; further, SE1457ΔsaeRS also released more extracellular DNA (eDNA) in the biofilms. Correlated with the increased autolysis phenotype, the transcription of autolysis-related genes, such as atlE and aae, was increased in SE1457ΔsaeRS. Whereas the expression of accumulation-associated protein was up-regulated by 1.8-fold in 1457ΔsaeRS, the expression of an N-acetylglucosaminyl transferase enzyme (encoded by icaA) critical for polysaccharide intercellular adhesin (PIA) synthesis was not affected by the deletion of saeRS. Conclusions Deletion of saeRS in S. epidermidis resulted in an increase in biofilm-forming ability, which was associated with increased eDNA release and up-regulated Aap expression. The increased eDNA release from SE1457ΔsaeRS was associated with increased bacterial autolysis and decreased bacterial cell viability in the planktonic/biofilm states.
- Subjects :
- DNA, Bacterial
Microbiology (medical)
Autolysis (biology)
lcsh:QR1-502
Virulence
Biology
DNA, Bacterial/metabolism
Transcription Factors/genetics/metabolism
medicine.disease_cause
Microbiology
lcsh:Microbiology
Bacterial cell structure
Bacteriolysis
Staphylococcus epidermidis/genetics/growth & development/physiology
Bacterial Proteins
Staphylococcus epidermidis
medicine
Biofilms/growth & development
Viability assay
ddc:616
Microbial Viability
Genetic Complementation Test
Biofilm
Gene Expression Regulation, Bacterial
biology.organism_classification
Complementation
Staphylococcus aureus
Biofilms
Bacterial Proteins/genetics/metabolism
Gene Deletion
Signal Transduction
Transcription Factors
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14712180
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Microbiology
- Accession number :
- edsair.doi.dedup.....7d721efd71c3110ec378a28d5db8a0b1
- Full Text :
- https://doi.org/10.1186/1471-2180-11-146