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Niosomal approach to brain delivery: Development, characterization and in vitro toxicological studies
- Source :
- International Journal of Pharmaceutics. 511:969-982
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- The majority of active agents do not readily permeate into brain due to the presence of the blood-brain barrier and blood-cerebrospinal fluid barrier. Currently, the most innovative and promising non-invasive strategy in brain delivery is the design and preparation of nanocarriers, which can move through the brain endothelium. Niosomes can perform brain delivery, in fact polysorbates, can act as an anchor for apolipoprotein E from blood plasma. The particles mimic LDL and interact with the LDL receptor leading to the endothelial cells uptake. The efficacy of niosomes for anticancer therapeutic applications was correlated to their physicochemical and drug delivery properties. Dimensions and ζ-potential were characterized using dynamic light scattering and asymmetric flow-field fractionation system. Lipid bilayer was characterized measuring the fluidity, polarity and microviscosity by fluorescent probe spectra evaluation. Morphology and homogeneity were characterized using atomic force microscopy. Physicochemical stability and serum stability (45% v/v fetal bovine and human serum) were evaluated as a function of time using dynamic light scattering. U87-MG human glioblastoma cells were used to evaluate vesicle cytotoxicity and internalisation efficiency. From the obtained data, the systems appear useful to perform a prolonged (modified) release of biological active substances to the central nervous system.
- Subjects :
- Pharmaceutical Science
Nanotechnology
02 engineering and technology
010402 general chemistry
Blood–brain barrier
01 natural sciences
Microviscosity
Drug Delivery Systems
Cell Line, Tumor
medicine
Animals
Humans
Niosome
Lipid bilayer
Serum Albumin
Liposome
Chemistry
Vesicle
Brain
021001 nanoscience & nanotechnology
0104 chemical sciences
medicine.anatomical_structure
Blood-Brain Barrier
Liposomes
Drug delivery
Biophysics
Cattle
Nanocarriers
0210 nano-technology
Subjects
Details
- ISSN :
- 03785173
- Volume :
- 511
- Database :
- OpenAIRE
- Journal :
- International Journal of Pharmaceutics
- Accession number :
- edsair.doi.dedup.....7d8c4ff77f966beef6cab2bbe20948de