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Enhanced gene delivery of low molecular weight PEI by flower-like ZnO microparticles
- Source :
- Materials Science and Engineering: C. 69:1367-1372
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Low molecular weight (1.8 kDa) branched polyethylenimine (PEI) has been used as non-viral vector for gene delivery because of its low toxicity, however, its further application in biomedical field has been restricted due to its low gene transfection efficiency. Herein, ZnO microflowers were prepared to increase the gene expression level mediated by PEI. Four methods have been applied to tune the shape of ZnO microstructures. Scanning electron microscopy (SEM) demonstrated the successful preparation of four kinds of flower like ZnO microparticles. By loading PEI/pDNA into ZnO microparticles, the formed new complexes showed enhanced gene transfection compared to PEI/pDNA alone. Cell uptaking experiments explained a possible mechanism that the tips of ZnO microflowers penetrated into the surface of cells, thus facilitating the entry of gene cargo into cells. These findings highlight the potential of needle like microstructure as adjuvant for efficient biomacromolecular delivery.
- Subjects :
- Materials science
Cell Survival
Scanning electron microscope
Metal Nanoparticles
Bioengineering
Nanotechnology
macromolecular substances
02 engineering and technology
Gene delivery
Transfection
010402 general chemistry
01 natural sciences
Biomaterials
chemistry.chemical_compound
X-Ray Diffraction
Gene expression level
Humans
Polyethyleneimine
Electrophoresis, Agar Gel
Polyethylenimine
Cell Death
Low toxicity
Flower like
Gene Transfer Techniques
DNA
021001 nanoscience & nanotechnology
Microstructure
Endocytosis
0104 chemical sciences
Molecular Weight
chemistry
Mechanics of Materials
Biophysics
Zinc Oxide
0210 nano-technology
HeLa Cells
Plasmids
Subjects
Details
- ISSN :
- 09284931
- Volume :
- 69
- Database :
- OpenAIRE
- Journal :
- Materials Science and Engineering: C
- Accession number :
- edsair.doi.dedup.....7d944d1a0e2a6de1b683a888e9b8cbb3