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IFN- gene expression is controlled by the architectural transcription factor HMGA1
- Source :
- International Immunology. 17:297-306
- Publication Year :
- 2005
- Publisher :
- Oxford University Press (OUP), 2005.
-
Abstract
- We report for the first time that IFNG gene expression requires high mobility group (HMG)A1, the architectural transcription factor mediating enhanceosome formation. This finding is supported by our direct studies of T cells isolated from the HMGA1-transgenic mice displaying an up-regulation of IFN-gamma production and of HMGA1-deficient mice exhibited a decreased IFN-gamma induction. In parallel transfection studies in EL4 cells, we observed elevated IFNG gene promoter activity in cells stably over-expressing HMGA1 and a reduction of such activity in cells expressing dominant-negative HMGA1. In vitro binding assays further demonstrated a specific interaction of HMGA1 to defined regions of the IFNG gene proximal promoter.
- Subjects :
- HMGA1
Transcription, Genetic
T-Lymphocytes
Molecular Sequence Data
Immunology
Lymphocyte Activation
Enhanceosome
Interferon-gamma
Mice
Transcription (biology)
Gene expression
Animals
Immunology and Allergy
Promoter Regions, Genetic
Transcription factor
HMGA Proteins
Regulation of gene expression
Base Sequence
biology
Promoter
General Medicine
Transfection
IFNG
Molecular biology
Recombinant Proteins
Gene Expression Regulation
biology.protein
transcription
Transcription Factors
Subjects
Details
- ISSN :
- 14602377
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- International Immunology
- Accession number :
- edsair.doi.dedup.....7d9c9d7e8d96d5b22d22ae9df221bb61