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Effect of FHA and Prn on Bordetella pertussis colonization of mice is dependent on vaccine type and anatomical site
- Source :
- PLoS ONE, Vol 15, Iss 8, p e0237394 (2020), PLoS One, 15, 8, PLoS ONE, PLoS One, 15
- Publication Year :
- 2020
- Publisher :
- Public Library of Science (PLoS), 2020.
-
Abstract
- Contains fulltext : 225116.pdf (Publisher’s version ) (Open Access) Bordetella pertussis vaccine escape mutants that lack expression of the pertussis antigen pertactin (Prn) have emerged in vaccinated populations in the last 10-20 years. Additionally, clinical isolates lacking another acellular pertussis (aP) vaccine component, filamentous hemagglutinin (FHA), have been found sporadically. Here, we show that both whole-cell pertussis (wP) and aP vaccines induced protection in the lungs of mice, but that the wP vaccine was more effective in nasal clearance. Importantly, bacterial populations isolated from the lungs shifted to an FHA-negative phenotype due to frameshift mutations in the fhaB gene. Loss of FHA expression was strongly selected for in Prn-deficient strains in the lungs following aP but not wP vaccination. The combined loss of Prn and FHA led to complete abrogation of bacterial surface binding by aP-induced serum antibodies. This study demonstrates vaccine- and anatomical site-dependent adaptation of B. pertussis and has major implications for the design of improved pertussis vaccines.
- Subjects :
- Bacterial Diseases
0301 basic medicine
Bordetella pertussis
Whooping Cough
Bordetella
Physiology
Mutant
Filamentous haemagglutinin adhesin
lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4]
Pathology and Laboratory Medicine
Biochemistry
Mice
Medical Conditions
Spectrum Analysis Techniques
0302 clinical medicine
Immune Physiology
Medicine and Health Sciences
Public and Occupational Health
Virulence Factors, Bordetella
030212 general & internal medicine
Lung
Vaccines
Immune System Proteins
Multidisciplinary
biology
Vaccination
Flow Cytometry
Antibodies, Bacterial
Vaccination and Immunization
Bacterial Pathogens
Hemagglutinins
Infectious Diseases
Medical Microbiology
Spectrophotometry
Medicine
Cytophotometry
Pathogens
Anatomy
Pertactin
Antibody
Bacterial Outer Membrane Proteins
Research Article
Infectious Disease Control
Science
Immunology
030106 microbiology
Nose
Diphtheria-Tetanus-acellular Pertussis Vaccines
Research and Analysis Methods
Microbiology
Antibodies
Frameshift mutation
03 medical and health sciences
Pertussis
Immunity
Animals
Microbial Pathogens
Bacteria
Organisms
Biology and Life Sciences
Proteins
biology.organism_classification
Gene Expression Regulation
Face
biology.protein
Preventive Medicine
Head
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 15
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....7da0ffd2f6f97b05bfcec57fd5b39437