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Heterologous Expression of Poplar WRKY18/35 Paralogs in Arabidopsis Reveals Their Antagonistic Regulation on Pathogen Resistance and Abiotic Stress Tolerance via Variable Hormonal Pathways
- Source :
- International Journal of Molecular Sciences, Volume 21, Issue 15, International Journal of Molecular Sciences, Vol 21, Iss 5440, p 5440 (2020)
- Publication Year :
- 2020
- Publisher :
- MDPI, 2020.
-
Abstract
- WRKY transcription factors (WRKY TFs) are one of the largest protein families in plants, and most of them play vital roles in response to biotic and abiotic stresses by regulating related signaling pathways. In this study, we isolated two WRKY TF genes PtrWRKY18 and PtrWRKY35 from Populustrichocarpa and overexpressed them in Arabidopsis. Expression pattern analyses showed that PtrWRKY18 and PtrWRKY35 respond to salicylic acid (SA), methyl JA (MeJA), abscisic acid (ABA), B. cinereal, and P. syringae treatment. The transgenic plants conferred higher B. cinerea tolerance than wild-type (WT) plants, and real-time quantitative (qRT)-PCR assays showed that PR3 and PDF1.2 had higher expression levels in transgenic plants, which was consistent with their tolerance to B. cinereal. The transgenic plants showed lower P. syringae tolerance than WT plants, and qRT-PCR analysis (PR1, PR2, and NPR1) also corresponded to this phenotype. Germination rate and root analysis showed that the transgenic plants are less sensitive to ABA, which leads to the reduced tolerance to osmotic stress and the increase of the death ratio and stomatal aperture. Compared with WT plants, a series of ABA-related genes (RD29A, ABO3, ABI4, ABI5, and DREB1A) were significantly down-regulated in PtrWRKY18 and PtrWRKY35 overexpression plants. All of these results demonstrated that the two WRKY TFs are multifunctional transcription factors in plant resistance.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Arabidopsis
Genetically modified crops
01 natural sciences
lcsh:Chemistry
chemistry.chemical_compound
PtrWRKY18
Gene Expression Regulation, Plant
lcsh:QH301-705.5
Abscisic acid
multifunctional
water-deficit
Spectroscopy
Disease Resistance
Plant Proteins
biology
food and beverages
General Medicine
NPR1
Plants, Genetically Modified
Computer Science Applications
Cell biology
Populus
Salicylic Acid
Signal Transduction
Germination
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
PtrWRKY35
Stress, Physiological
Physical and Theoretical Chemistry
Molecular Biology
Plant Diseases
Abiotic stress
Arabidopsis Proteins
Organic Chemistry
fungi
biology.organism_classification
WRKY protein domain
PtrWRKY18 and PtrWRKY35
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
chemistry
pathogen resistance
Heterologous expression
Salicylic acid
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 21
- Issue :
- 15
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....7e253a1ae5ec7b4353ca7dc289bdcc1c