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Single cell RNA sequencing of stem cell-derived retinal ganglion cells
- Source :
- Scientific Data
- Publication Year :
- 2017
-
Abstract
- We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ganglion cells (RGCs) at the single cell level. The human embryonic stem cell line BRN3B-mCherry (A81-H7), was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster of 288 cells showed increased expression of genes involved in axon guidance together with semaphorin interactions, cell-extracellular matrix interactions and ECM proteoglycans, suggestive of a more mature RGC phenotype.
- Subjects :
- 0301 basic medicine
Statistics and Probability
Retinal Ganglion Cells
Data Descriptor
Cellular differentiation
Biology
Library and Information Sciences
Retinal ganglion
Education
Cell Line
03 medical and health sciences
0302 clinical medicine
Single-cell analysis
Humans
Transcriptomics
Embryonic Stem Cells
Neural stem cells
Base Sequence
Sequence Analysis, RNA
Cell Differentiation
Molecular biology
Embryonic stem cell
Cell biology
Computer Science Applications
030104 developmental biology
Single cell sequencing
Optic nerve diseases
RNA
sense organs
Stem cell
Statistics, Probability and Uncertainty
Single-Cell Analysis
030217 neurology & neurosurgery
Adult stem cell
Human embryonic stem cell line
Information Systems
Subjects
Details
- ISSN :
- 20524463
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Scientific data
- Accession number :
- edsair.doi.dedup.....7e57cc40681c66b8b0dd1adfbb75644f